Coxiella burnetii is an intracellular bacterium which causes Q-fever. The genomes from a number of isolates obtained from the Dutch outbreak, were sequenced. These genome sequences form the basis for improved typing methods, enabling more accurate source finding and epidemiological studies. In addition, the genome sequences are highly valuable for other research, for instance of the relation between the Dutch outbreak and altered virulence properties. As a result of this project, 19 isolates from the Dutch outbreak have been cultivated. These isolates were primarily obtained from veterinary sources, but a few were acquired from human or environmental sources. The genomes of 3 isolates have been almost completely sequenced and some preliminary analyses were performed. Raw sequences from 4 additional isolates will be available soon. The bacterial isolates and genome sequences play important roles in several ongoing projects from the research partners. The experience that was acquired with harvesting and sequencing C. burnetii genomes is highly valuable for current and future research of C. burnetii and other intracellular pathogens.
