Vaessen HAMG ,
Loon JW van ,
Tolsma K ,
Ooik A van
45 p
in Dutch
1994
Toon Nederlands
English Abstract This report describes the development and the
performance characteristics of the methods of analysis for lead in blood and
for cadmium and mercury in urine. The methods are designed to assess
samples of segments of the Dutch population not specifically exposed to
these analytes. For lead and cadmium samples are wet pressurized digested
with nitric acid at 150 degrees C and then assayed for these elements with
flameless atomic absorption spectroscopy and Zeeman background correction
after atomisation of the sample solution from a L'vov platform ; Annexes I
and II. Validation of the lead in blood procedure was done by assaying
reference material CRM 194 "Lyophilized Bovine Blood". For cadmium in urine
the reference material "Kontroll-Urin for Metalle 2" was used and recovery
experiments were carried out for validation. The limit of determination for
lead is 8 mug/l for a test portion of 1 ml and for cadmium in urine 0.045
mug/l when 3 ml is used for analysis. Sample preparation by wet pressurized
digestion with nitric acid, now at 120 degrees C, also is used to determine
mercury in urine. The resulting test solution is assayed for mercury by
cold vapor atomic absorption spectroscopy ; Annex III. Validation of the
method was done by recovery experiments. For a test portion of 4 g urine
the limit of determination is 0.03 mug/kg.
