Comparative study of the microbidical activity of alcohols under the conditions of the European Suspension Test
Vergelijkend onderzoek naar de desinfecterende werking van alcoholen in de Europese Suspensie Test
Pullen W , Klingeren B van
RIVM Report 358704003
To obtain quantitative data on the microbidical activity of ethanol, n-propanol and isopropanol under the conditions of the European Suspension Test, comparative tests were carried out in the concentration range of 20 to 80% v/v using the standard test organisms of this test methods (4 bacteria and 1 yeast) as well as a strain of Mycobacterium terrae and spores of Aspergillus niger. The results show that all alcohols rested are rapidly cidal against bacteria and yeasts, inducing the norm reduction of 5 log cycles within 2 minutes. Propanols, especially n-propanol, are slightly more active than ethanol, reaching the above reduction already at concentrations of 30% v/v. Spores of A.niger are less susceptible. At the usual concentration of 70% v/v only ethanol causes within 5 minutes the desired reduction of 5 log ; with n-propanol and isopropanol the reduction is limited to approximately 4 and 2 log reduction respectively.