[Extension of second messengers: phosphoinositolsystem. Final report on pilotexperiments.]
Uitbreiding second messengers: fosfoinositolsysteem. Eindrapportage over pilotexperimenten
Bisschop A, Eigeman L, van Amsterdam JGC, Wemer J
RIVM Report 319001004
A pilot study was conducted in order to investigate the possibility to measure the IP3 (inositoltrisphosphate) content in cardiovascular tissue of the rat, by means of a radioimmunoassay. With the kit is was possible to obtain a linear standard calibration curve. In extracts of control- and 10 mM NaF stimulated aorta's and left ventricles it appeared that with the applied method the measurement of IP3 was unsuccessful. In contrast to the sample preparation method, the measurement assay is not problematic. It was decided not to continue further investigations.