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SFE, in combination with carbon trapping, for the analysis of dioxins and planar PCBs in fish

SFE, in combinatie met adsorptie aan kool voor de analyse van dioxines en planaire PCB's in vis

Synopsis

This report describes the results of an exchange project in the framework of the Human Capital Mobility Programme (HCMP). The work was performed at the Joint Research Centre (JRC) in Ispra, Italy. The goal of the project was to develop a method for the off-line supercritical fluid extraction and separation of dioxins and planar PCBs in fish with active carbon used as trapping material. JRC was supporting experiences with the SFE of PCBs in fatty matrices and RIVM-LOC was supporting experiences with the SFE of PCBs in soil and the knowledge about the conventional extraction of dioxins and planar PCBs in all kind of matrices. The development of the carbon trapping was performed in three steps. First the off-line determination of the minimal amount of carbon necessary to trap dioxins and planar PCBs. The second step was to use the trap of the instrument to trap the components in a semi on-line mode. The third step was to investigate the SFE technique with fatty matrices and to check if it is possible to trap the components after SFE. A large difference in the amounts of carbon necessary to trap and separate the components was found, going from off-line via semi on-line to on-line trapping. After modification of the SFE conditions it was possible to extract nearly the total amount of fat from the freeze dried herringfilet and about 70 till 90 % of the components. A linear restrictor did not seem to be suitable for the supercritical fluid extraction of fatty matrices, because of clogging problems. The project has resulted in an better understanding in the possibilities and use of SFE for the extraction of organochlorine components from fatty matrices. The project will be continued using a more suitable SFE instrument.
 

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