Demonstration of viruses in water: comparison of the detection sensitivity of the immunoperoxidase assay and the plaque overlay assay
Aantonen van virussen in water: een vergelijking van de detectiegevoeligheid van de immunoperoxidase methode met de plaque overlay methode
Theunissen JJH , Krebbers AHW , Medema GJ
RIVM Report 289202010
The testing of a method to increase the sensitivity of the current culture method used until now has been described. A sensitivity increase will lead to a better estimation of the virus content of surface water and to a better insight in measurements that should be taken to minimise the infection risk represented by drinking water or bathing water. In six out of 12 naturally contaminated water concentrates the virustiters found by the immunoperoxidase method appeared to be higher, in five out of 12 the titers were comparable and in one out of 12 the titer was lower than that found when the plaque overlay was used. The overall difference was not statistically significant. So the desired increase in sensitivity of the immunoperoxidase assay compared to the plaque overlay assay could not be realised. Due to the minimal increase in sensitivity combined with the smaller sample volume that can be processed, the immunoperoxidase method can only be used when analysing water concentrates with a virus content of at least 1 virus per milliliter.