Short term toxicity of polycyclic aromatic hydrocarbons (PAC) in flounder (Platichthys flesus): preliminary experiments with benzo[a]pyrene en 7,12-dimethylbenz[a]anthracene
Kortdurende toxiciteit van polycyclische aromatische koolwaterstoffen (PAK's) in de bot (Platichthys flesus): orienterende studies met benzo[a]pyreen en 7,12-dimethylbenz[a]anthraceen
Grinwis GCM, van den Brandhof EJ, Leewis RJ, Wester PW, Vaal MA, Vethaak AD, Vos JG
RIVM Report 703715002
In this report 5 pilot studies on short term exposure of flounder (Platichthys flesus) to benzo[a]pyrene (BaP) and 7,12-dimehtylbenz[a]anthracene (DMBA) under controlled laboratory conditions are described. Exposure took place with high concentrations through the water-phase and by intraperitoneal injection. Effects on behaviour and exterior were checked, and the histopathological effects on gills, thymus, hepatopancreas, digestive tract, pro- and mesonephros, spleen, and ovary or testicles were investigated. We also looked for the induction of the enzyme cytochrome P450 1A1 (CYP 1A1) as a measure for the biotransformation of PAH's and the formation of biologically active metabolites. Exposure of flounder to the PAH's did not result in striking effects in spite of the high concentrations. Only a slight increase in immunoreactivity for CYP 1A1 in hepatocytes of the injected animals was noted. Flounder appears to be not very sensitive to PAHs under the used experimental conditions. Therefore PAHs may not play a very important role in the induction of diseases in flounder as seen in the field.
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