Validation of a liquid handling system for use in clinical vaccine studies performed by LVO; Implementation of the MultiPROBE 104/204 Canberra Packard
Validatie van een pipetteerstation voor gebruik bij klinische vaccin studies uitgevoerd door LVO; Implementatie van de MultiPROBE 104/204 Canberra Packard
van Gageldonk PGM, Berbers GAM
RIVM Report 126000002
The MultiPROBE type 104/204 manufactured by Canberra Packard is a liquid handling system for sample processing in clinical vaccine studies within LVO. The apparatus is used for transfer of sera and sample dilutions in several antibody assays, especially ELISA's. These liquid handlings with the MultiPROBE are compared with manual sample handling. Experiments show that introduction of the MultiPROBE for use in clinical studies as a substitute for manual handling did not result in systematical and significant changes in antibody assay results. The correlation between both methods for one step dilutions as well as for serial dilutions is (very) good. Reproducibility of the MultiPROBE is comparable and sometimes even better than manual handling.