EU Interlaboratory comparison study veterinary-X (2006) Bacteriological detection of Salmonella in pig faeces
EU Ringonderzoek veterinair-X (2006) Bacteriologische detectie van Salmonella in varkensfaeces
26 May 2012, PDF |
85 pages |
Kuijpers AFA, Veenman C, Mooijman KA
RIVM Report 330604004
The European National Reference Laboratories (NRLs) for Salmonella were able to detect high and low levels of Salmonella in pig faeces. This is shown in the 10th interlaboratory comparison study, in which 27 laboratories participated. The goal of the study, organised by the Community Reference Laboratory (CRL) for Salmonella in November 2006, was to see if the participating laboratories could detect Salmonella in pig faeces. Each laboratory received a package containing pig faeces and 35 gelatin capsules displaying different levels of Salmonella. The instructions to the laboratories were to spike the pig faeces with the capsules and test these samples for the presence of Salmonella, which they did. The laboratories used one prescribed method for running this test, namely, a selective culture step on Modified Semi-solid Rappaport Vassiliadis (MSRV). The laboratories found Salmonella in 97% of the samples. Three laboratories had problems detecting Salmonella in the samples with low levels of contamination. The CRL contacted these laboratories to request an explanation for the deviating results; CRL also offered the possibility of performing extra analyses. These three laboratories too achieved a good performance in the follow-up study in February 2007.