Fifteenth EURL-Salmonella interlaboratory comparison study (2010) on typing of Salmonella ssp.
Vijftiende EURL-Salmonella ringonderzoek (2010) voor de typering van Salmonella spp.
26 May 2012, PDF |
94 pages |
Pol-Hofstad IE, Jacobs-Reitsma WF, Maas HME, de Pinna E, Mooijman KA
RIVM Report 330604024
The National Reference Laboratories (NRLs) of all 27 European Member States, as well as the NRLs of Croatia, Former Yugoslav Republic of Macedonia, Norway, Turkey and Switzerland performed well on the 2010 quality control test on Salmonella typing. Four laboratories were found to require a follow-up study on their first test but all participants obtained good scores in this follow-up study. An analysis of the pooled results from all NRLs revealed that the NRLs taken as a whole were able to assign the correct name to 95% of the strains tested. Since 1992, the NRLs of the EU Member States have been required to participate in annual quality control tests, which consist of interlaboratory comparison studies on Salmonella. Each Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of these NRLs on Salmonella typing is assessed annually, based on their capability to correctly identify 20 Salmonella strains. NRLs from countries outside the European Union occasionally participate in these tests on a voluntary basis. Croatia, Former Yugoslav Republic of Macedonia, Norway, Turkey and Switzerland took part in the 2010 test. Seven NRLs not only serotyped the 20 Salmonella strains of the quality control test, but also subtyped 20 additional strains by phage typing. For this, the laboratories received ten strains of Salmonella Enteritidis and ten strains of Salmonella Typhimurium. These NRLs typed 98% of the S. Typhimurium strains correctly. Of the S. Enteritidis strains, 99% were phage typed correctly. The European Union Reference Laboratory for Salmonella (EURL-Salmonella) organizes this annual interlaboratory comparison study on typing of Salmonella in cooperation with the Health Protection Agency in London, UK. The EURLSalmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.