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Kamerman JW , Gestel CAM van

54 p in Dutch   1991

Toon Nederlands

English Abstract
In this report a description is given of the (further) development of bioassays for estimating the bioavailability of metals in soils using earthworms (Eisenia fetida), lettuce (Lacuca sativa) and Radish (Raphanus sativus). The bioassays can be used in unpolluted, contaminated and cleaned-up soils. In former research soils cleaned-up by extraction appeared to have lost structure and nutrients so that earthworms died and plants didn't grow. A standard additive was developed which enables the performance of the bioassays in both contaminated and cleaned-up soils without, influencing the bioavailability of the metals. Therefore several additives (peat, clay, sand, perlite, cow-dung and nutrient solution) in varying quantities were tested in some contaminated and cleaned-up soils. The influence of the additives on the survival of worms and the growth of radish and lettuce were established. The bioassays lasted 4 weeks. A suited standard additive for the bioassay using earthworms appeared to be: 8 vol% perlite ; 1 g cow-dung/ worm ; nutrient solution. The developed standard additive for the bioassays using radish or lettuce consists of: 8 vol% perlite ; nutrient solution. The standard additives were tested in contaminated and cleaned-up soils and appeared to enable survival of the earthworms and proper growth of radish and lettuce. The additives did not influence the bioavailability of the metals in these soils.


RIVM - Bilthoven - the Netherlands - www.rivm.nl

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RIVM - Bilthoven - Nederland - www.rivm.nl

( 1991-07-31 )