Stolker AAM ,
Bode W ,
Toet AE ,
Wemer J ,
Ginkel LA van
27 p
in Dutch
1993
Toon Nederlands
English Abstract This report describes a method for the determination of
the enantiomers of the beta-blocker propranolol. Propranolol is isolated by
solid phase extraction. The propranolol enantiomers are derivatized to
diastereomeric compounds by reaction with the chiral reagent
(-)-menthylchloroformate. The diastereoisomers are separated by
reversed-phase high-performance liquid chromatography (HPLC) on an
octadecylcolumn, using methanol-water (875/125 ; v/v) as eluent. The
diastereoisomers are detected by fluorescence detection. Standard curves
for both R-(+)-propranolol and S-(-)-propranolol are linear in the
0,050-1,50 mg/l range. The limit of detection is 0,010 mg/l for each
enantiomer. The limit of determination is 0,050 mg/l for each enantiomer
based on a 50 mul plasma. The extraction recoveries are between 95-100%.
Within-laboratory reproducibility (n=20) is 5,5% for S-(-)-propranolol and
5,9% for R-(+)-propranolol at 0,352 mg/l. The method has been used for
pharmacokinetic studies of propranolol enantiomers in
rats.
