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Heeft E van der , Jong APJM de

18 p in Dutch   1993

Toon Nederlands

English Abstract
This report describes the results of an investigation into the utility of electrospray ionization - mass spectrometry (ESI-MS) for the determination of the molecular mass of small proteins (up to 17 kDa). The molecular mass of four pure proteins, was determined by ESI-MS. From the known molecular mass of the proteins, the accuracy of the method can be estimated. Two methods were applied to calculate the molecular mass from the acquired ESI mass spectra. In terms of accuracy and precision of the measured molecular mass, the two methods were almost equal. The results of this investigation show that an accuracy of about 0.01% and precision of 0.006% can be achieved. The acquisition of the ESI mass spectro required 10 pmol of protein.


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RIVM - Bilthoven - Nederland - www.rivm.nl

( 1993-10-31 )