English Abstract This report describes the results of a study on the
development of an interface for the coupling of narrow-bone reversed-phase
liquid chromatography (RPLC) and Fourier transform infrared spectrometry
(FTIR). The interface is designed to evaporate the mobile phase by means of
a spray jet assembly under simultaneous deposition of the eluting compounds
onto a linearly moving IR transparant substrate. Subsequently the
immobilized chromatogram is scanned by FTIR microscopy. Polycyclic
hydrocarbons and quinones have been used as model compounds. The
chromatographic seperation of these compounds is found to be hardly affected
by the immobilization process. Transmission measurement using zinc selenide
as a substrate appeared to be preferable over measurement in the reflection
mode on an aluminum surface. Identificaiton limits are in the 10-20 ng
range (injected). The interface is able to handle eluents containing up to
40% water.
