Bioassays for tetrachloro-dibenzo-p-dioxin 730501050

Bioassays for tetrachloro-dibenzo-p-dioxin

[ [Bioassays voor tetrachloor-dibenzo-p-dioxine.] ]

Jansen EHJM, Laan CA, van den Berg RH, van Iersel AAJ, Zomer G

20 p in English 1992

RIVM Rapport 730501050

Toon Nederlands

English Abstract
In this report three independent bioassays for the determination of dioxinlike compounds have been evaluated. These assays include an immunochemical determination of 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD), an inhibition of estrogen-induced cathepsin D in MCF-7 cell line and the induction of cytochrome P-450 IA1 in isolated hepatocytes. It appeared that in our experiments only the last bioassay was successful. A limit of detection of 2 pg TCDD/assay was achieved with the cultured hepatocytes from rats. Thus far only standard solutions have been tested without the influence of matrix components of environmental or biological samples or the influence of other contaminants. The assay can be automated and performed in relatively large numbers.

RIVM - Bilthoven - the Netherlands - www.rivm.nl

Rijksinstituut voor Volksgezondheid en Milieu RIVM
_{( 1992-12-31 )}