Test results of Salmonella sero- and phage typing by the National Reference Laboratories in the Member States of the European Union
Test resultaten van Salmonella sero- en faag typering door Nationale Referentie Laboratoria in de lidstaten van de Europese Unie
26 May 2012, PDF |
64 pages |
Raes M , Ward LR , Maas HME , Wannet WJB , Henken AM
RIVM Report 284500016
The fifth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven) in collaboration with the Public Health Laboratory Services (PHLS, London). All 17 National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs-Salmonella are also ENL. The results of these three laboratories will be evaluated with the NRLs-Salmonella as well as with the ENLs. In total, 20 strains of the species Salmonella enterica were selected for serotyping and antibiotic resistance pattern typing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, with the typing of the H antigens some laboratories had problems. Antibiotic resistance pattern typing revealed data which show that standardisation of this technique is required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.