EU Interlaboratory comparison study animal feed I (2008). Bacteriological detection of Salmonella in chicken feed
EU Ringonderzoek dierenvoeder-I (2008). Bacteriologische detectie van Salmonella in kippenvoer
26 May 2012, PDF |
87 pages |
Kuipers AFA, Veenman C, van de Kassteele J, Mooijman KA
RIVM Report 330604012
In 2008, from the 30 National Reference Laboratories (NRLs) in the European Union, 29 were able to detect high and low levels of Salmonella in chicken feed. They achieved the desired outcome at once. One laboratory was unable to produce satisfactory results neither in the follow-up test. The reasons for their failures are currently being investigated. These results were shown in the first interlaboratory comparison study on animal feed, organized by the Community Reference Laboratory (CRL) for Salmonella. The comparison study was conducted in October 2008, with the follow up in March 2009. The NRLs responsible for Salmonella detection from all European Member States are obliged to participate in this study. The CRL for Salmonella is part of the Dutch National Institute for Public Health and the Environment (RIVM). During the study, three methods for detecting Salmonella in chicken feed were used. Two methods are standardised at international level for the detection of Salmonella in animal feed. One of those two methods was least effective as only 92 percent of the samples were found to be positive. The third, internationally prescribed method for the detection of Salmonella in veterinary samples was not obligatory but requested by the CRL. This method gave the best results with 99 percent being positive. To perform the test, the laboratories had to follow the given instructions. Each laboratory received a package containing chicken feed and 35 gelatin capsules containing Salmonella spp. at different levels. The laboratories were instructed to spike the chicken feed with the capsules and test the samples for the presence of Salmonella.