APROBA-Plus is a user friendly excel tool that facilitates probabilistic risk assessment of substances, including uncertainty analysis. The use of APROBA-Plus is recommended for practically all substance risk assessments. Its outcome helps risk managers to make decisions. The tool compares a high exposure to the dose which causes a particular adverse health effect in sensitive individuals. Rather than comparing (conservative) point estimates for the effect dose and exposure, the entire uncertainty distribution of both inputs is included in the analysis.