On this page, you will find an overview of RIVM publications related to alternatives to animal testing and safety assessment of chemicals and pharmaceuticals.
| Title | Authors | Journal |
|---|---|---|
| An adverse outcome pathway for chemical-induced Parkinson’s disease: Calcium is key | Meerman, J.J., Legler, J., Piersma, A.H., Westerink, R.H.S., Heusinkveld, H.J. | NeuroToxicology 2023: 99: 226-243 |
| The Importance of Variations in In VitroDosimetry to Support Risk Assessment of Inhaled Toxicants | Staal, Y.C.M., Geraets, L., Rothen-Rutishauer, B., Clift, M.J.D., Braakhuis, H., Kienhuis, A.S., Bos, P.J.M. | ALTEX, accepted manuscript, published October 12, 2023 |
| New approach methodologies to facilitate and improve the hazard assessment of non-genotoxic carcinogens—a PARC project | Audebert, M., Assmann, A.S., Azqueta, A., Babica, P. Benfenati, E., Bortoli, S. Heusinkveld, H.J., Luijten, M., et al. | Front Toxicol 2023; 5 |
| New approach methodologies in human regulatory toxicology – Not if, but how and when! | Schmeisser, S., Miccoli, A., Bergen, M. von, Berggren, E., Braeuning, A., Busch, W., Luijten, M., et al. | Environment Int 2023; 178:108082 |
| Variability of in vivo potency assays of whole-cell pertussis, inactivated polio, and meningococcal B vaccines | Walstijn, C. van, Verweij, S., Care, R., Rigsby, P., Clapper, E-B., Markey, K., Vandebriel, R.J., Stickings, P. Hoefnagel, M.H.N. | Vaccine 2023; 41(38):5603-5613 |
| Retinoic acid signaling pathway perturbation impacts mesodermal-tissue development in the zebrafish embryo: Biomarker candidate identification using transcriptomics | Samrani, L.M.M., Dumont, F., Hallmark, N., Bars, R., Tinwell, H., Pallardy, M., Piersma, A.H. | Regulatory Toxicology and Pharmacology 2023; 142:105438 |
| Transferability and reproducibility of exposed air-liquid interface co-culture lung models | Braakhuis, H.M., Gremmer, E.R., Bannuscher, A. Drasler, B., Keshavan, S., Rothen-Rutishauser, B., Birk, B., Verlohner, A. Vandebriel, R.J., et al. | NanoImpact 2023; 31:100466 |
| An adverse outcome pathway network for chemically-induced oxidative stress leading to (non-)genotoxic carcinogenesis | Veltman, C.H.J., Pennings, J.L.A., Water, B. van de, Luijten, M. | Chem Res Toxicol 2023; online ahead of print |
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The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design |
Ruijter, N., Soeteman-Hernandez, L.G., Carriere, M., Boyles, M., McLean, P., Catalan, J., et al. | Nanomaterials 2023; 13(3): 472 |
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Prolonged differentiation of neuron-astrocyte co-cultures results in emergence of dopaminergic neurons |
de Leeuw, V.C., van Oostrom, C.T.M., Zwart, E.P., Heusinkveld H.J., Hessel, E.V.S. | Int J Mol Sci 2023; 24(4): 3608 |
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Innovating human chemical hazard and risk assessment through a holistic approach |
Piersma, A.H., Heusinkveld, H., Hessel, E., Kienhuis, A. | Current Opinion in Toxicology 2023; 33: 100386 |
| Neurodegeneration in a regulatory context: The need for speed | Meerman, J. J., Wolterink, G., Hessel, E. V. S., de Jong, E., & Heusinkveld, H. J. | Current Opinion in Toxicology 2023; 33: 100383 |
| Application of AOPs to assist regulatory assessment of chemical risks – Case studies, needs and recommendations | Bajard, L., Adamovsky, O., Audouze, K., Baken, K., Barouki, R., Beltman, J. B., Luijten, et al. | Environmental Research 2023, 217:114650 |
| Dynamic regulation of gene expression and morphogenesis in the zebrafish embryo test after exposure to all-trans retinoic acid. | Samrani, L. M. M., Pennings, J. L. A., Hallmark, N., Bars, R., Tinwell, H., Pallardy, M., & Piersma, A. H. | Reproductive Toxicology 2023: 115:8-16 |
| Towards achieving a modern science-based paradigm for agrochemical carcinogenicity assessment. | Hilton, G. M., Corvi, R., Luijten, M., Mehta, J., & Wolf, D. C. | Regulatory Toxicology and Pharmacology 2023; 137:105301 |
| Title | Authors | Journal |
|---|---|---|
|
Smoking-associated exposure of human primary bronchial epithelial cells to aldehydes: impact on molecular mechanisms controlling mitochondrial content and function |
Tulen, C.B.M., Duistermaat, E., Cremers, J.W.J.M., Klerx, W.N.M., Fokkens, P.H.B., Weibolt, N., Kloosterboer, N., Dentener, M.A., Gremmer, E.R., Jessen, P.J.J., Koene, E.J.C., Maas, L., Opperhuizen, A., van Schooten, F.J., Staal, Y.C.M., Remels, A.H.V. | Cells 2022; 11(21): 3481 |
| The Current Status and Work of Three Rs Centres and Platforms in Europe | Neuhaus, W., Reininger-Gutmann, B., Rinner, B., Plasenzotti, R., Wilflingseder, D., De Kock, J., Vanhaecke, T., Rogiers, V., Jírová, D., Kejlova, K., Knudsen, L., Nielsen, R., Kleuser, B., Kral, V., Thöne-Reineke, C., Hartung, T., Pallocca, G., Rovida, C., Leist, M., & Spielmann, H. | Alternatives to laboratory animals : ATLA 2022: 50(6):381-413 |
| Applicability of generic PBK modelling in chemical hazard assessment: A case study with IndusChemFate. | Fragki, S., Piersma, A. H., Westerhout, J., Kienhuis, A., Kramer, N. I., & Zeilmaker, M. J. | Regulatory Toxicology and Pharmacology 2022; 136:105267 |
| Smoking-Associated Exposure of Human Primary Bronchial Epithelial Cells to Aldehydes: Impact on Molecular Mechanisms Controlling Mitochondrial Content and Function | Tulen, C. B. M., Duistermaat, E., Cremers, J. W. J. M., Klerx, W. N. M., Fokkens, P. H. B., Weibolt, N., Kloosterboer, N., Dentener, M. A., Gremmer, E. R., Jessen, P. J. J., Koene, E. J. C., Maas, L., Opperhuizen, A., van Schooten, F. J., Staal, Y. C. M., & Remels, A. H. V. | Cells 2022; 11(21):3481 |
| Analyses of Transcriptomics Cell Signalling for Pre-Screening Applications in the Integrated Approach for Testing and Assessment of Non-Genotoxic Carcinogens. | Oku, Y., Madia, F., Lau, P., Paparella, M., McGovern, T., Luijten, M., & Jacobs, M. N. | International Journal of Molecular Sciences 2022; 23(21):12718 |
| Neuronal differentiation pathways and compound-induced developmental neurotoxicity in the human neural progenitor cell test (hNPT) revealed by RNA-seq. | de Leeuw, V. C., van Oostrom, C. T. M., Wackers, P. F. K., Pennings, J. L. A., Hodemaekers, H. M., Piersma, A. H., & Hessel, E. V. S. | Chemosphere 2022: 304:135298 |
| Development of a cell line-based in vitro assay for assessment of Diphtheria, Tetanus and acellular Pertussis (DTaP)-induced inflammasome activation | Vandebriel, R. J., Stalpers, C. A. L., Vermeulen, J. P., Remkes, M., Schmelter, M., Broere, F., & Hoefnagel, M. H. N. | Vaccine 2022; 40(39):5601-7 |
| Prioritization of chemicals in food for risk assessment by integrating exposure estimates and new approach methodologies: A next generation risk assessment case study | Luijten, M., Sprong, R. C., Rorije, E., & van der Ven, L. T. M. | Frontiers in Toxicology 2022: 4 |
| Transcriptome and proteome analysis of innate immune responses to inactivated Leptospira and bivalent Leptospira vaccines in canine 030-D cells. | Novak, A., Pennings, J. L. A., van der Maas, L., Meiring, H. D., Ludwig, I., Verkoeijen, S., Rutten, V., Broere, F., & Sloots, A. | Scientific Reports 2022; 12(1):13418 |
| Next-generation risk assessment of chemicals – Rolling out a human-centric testing strategy to drive 3R implementation: The RISK-HUNT3R project perspective. | Pallocca, G., Moné, M. J., Kamp, H., Luijten, M., van de Water, B., & Leist, M. | ALTEX - Alternatives to animal experimentation 2022; 39(3):419-26 |
| Identification of proteome markers for drug-induced liver injury in zebrafish embryos. | Driessen, M., van der Plas - Duivesteijn, S., Kienhuis, A. S., van den Brandhof, E.-J., Roodbergen, M., van de Water, B., Spaink, H. P., Palmblad, M., van der Ven, L. T. M., & Pennings, J. L. A. | Toxicology 2022: 477:153262 |
| Development of a Roadmap for Action on New Approach Methodologies in Risk Assessment. | Escher, S. E., Partosch, F., Konzok, S., Jennings, P., Luijten, M., Kienhuis, A., de Leeuw, V., Reuss, R., Lindemann, K.-M., & Bennekou, S. H. | EFSA Supporting Publications 2022; 19(6):7341E |
| Dose Addition in the Induction of Craniofacial Malformations in Zebrafish Embryos Exposed to a Complex Mixture of Food-Relevant Chemicals with Dissimilar Modes of Action. | Van Der Ven Leo, T. M., Van Ommeren, P., Zwart Edwin, P., Gremmer Eric, R., Hodemaekers Hennie, M., Heusinkveld Harm, J., van Klaveren Jacob, D., & Rorije, E. | Environmental Health Perspectives 2022; 130(4):047003 |
| Potential of concentration-response data to broaden regulatory application of in vitro test guidelines. | Jacobs, M. N., Ezendam, J., Hakkert, B., & Oelgeschlaeger, M. | ALTEX - Alternatives to animal experimentation 2022; 39(2):315-21 |
| The Rise of Three Rs Centres and Platforms in Europe | Neuhaus, W., Reininger-Gutmann, B., Rinner, B., Plasenzotti, R., Wilflingseder, D., , Kienhuis, A., et al. | Alternatives to laboratory animals : ATLA 2022; 50(2):90-120 |
| AOP report: Development of an adverse outcome pathway for oxidative DNA damage leading to mutations and chromosomal aberrations | Cho, E., Allemang, A., Audebert, M., Chauhan, V., Dertinger, S., Hendriks, G., Luijten, M., Marchetti, F., Minocherhomji, S., Pfuhler, S., Roberts, D. J., Trenz, K., & Yauk, C. L. | Environmental and Molecular Mutagenesis 2022; 63(3):118-34 |
| Pluripotent stem cell assays: Modalities and applications for predictive developmental toxicity | Piersma, A. H., Baker, N. C., Daston, G. P., Flick, B., Fujiwara, M., Knudsen, T. B., Spielmann, H., Suzuki, N., Tsaioun, K., & Kojima, H. | Current Research in Toxicology 2022: 3:100074 |
| Regulation of Clostridium tetani Neurotoxin Expression by Culture Conditions. | Pennings, J. L. A., Abachin, E., Esson, R., Hodemaekers, H., Francotte, A., Claude, J.-B., Vanhee, C., Uhlrich, S., & Vandebriel, R. J. | Toxins 2022; 14(1) |
| Integrating in vitro chemical transplacental passage into a generic PBK model: A QIVIVE approach | Fragki, S., Hoogenveen, R., van Oostrom, C., Schwillens, P., Piersma, A. H., & Zeilmaker, M. J. | Toxicology 2022; 465:153060 |
| Neuromodulatory and neurotoxic effects of e-cigarette vapor using a realistic exposure method | Staal, Y. C. M., Li, Y., Gerber, L.-S., Fokkens, P., Cremers, H., Cassee, F. R., Talhout, R., Westerink, R. H. S., & Heusinkveld, H. J. | Inhalation Toxicology 2022:1-10 |
| Title | Authors | Journal |
|---|---|---|
| Integrating 3Rs approaches in WHO guidelines for the batch release testing of biologicals. | Lilley, E., Isbrucker, R., Ragan, I., & Holmes, A. | Biologicals 2021; 74:24-7 |
| Exploring Neurobehaviour in Zebrafish Embryos as a Screening Model for Addictiveness of Substances | Havermans, A., Zwart, E. P., Cremers, H. W. J. M., van Schijndel, M. D. M., Constant, R. S., Mešković, M., et al. | Toxics 2021; 9(10:250 |
| Developmental Neurotoxicity of Environmentally Relevant Pharmaceuticals and Mixtures Thereof in a Zebrafish Embryo Behavioural Test | Atzei, A., Jense, I., Zwart, E. P., Legradi, J., Venhuis, B. J., van der Ven, L. T. M., Heusinkveld, H. J., Hessel, E. V. S. | International Journal of Environmental Research and Public Health 2021; 18(13):6717 |
| Safer chemicals using less animals: kick-off of the European ONTOX project | Vinken, M., Benfenati, E., Busquet, F., Castell, J., Clevert, D. A., de Kok, , et al. | Toxicology 2021; 458:152486 |
| Optimization of an air-liquid interface in vitro cell co-culture model to estimate the hazard of aerosol exposures | He, R. W., Braakhuis, H. M., Vandebriel, R. J., Staal, Y. C. M., Gremmer, E. R., Fokkens, , et al. | Journal of aerosol science 2021; 153:105703 |
| An ontology for developmental processes and toxicities of neural tube closure. | Heusinkveld, H. J., Staal, Y. C. M., Baker, N. C., Daston, G., Knudsen, T. B., & Piersma, A. H. | Reproductive Toxicology 2021; 99:160-7 |
| Title | Authors | Journal |
|---|---|---|
| An efficient neuron-astrocyte differentiation protocol from human embryonic stem cell-derived neural progenitors to assess chemical-induced developmental neurotoxicity. | de Leeuw, V. C., van Oostrom, C. T. M., Westerink, R. H. S., Piersma, A. H., Heusinkveld, H. J., & Hessel, E. V. S. | Reproductive Toxicology 2020; 98:107-16 |
| The effects of aliphatic alcohols and related acid metabolites in zebrafish embryos - correlations with rat developmental toxicity and with effects in advanced life stages in fish | van der Ven, L. T. M., Schoonen, W. G., Groot, R. M., den Ouden, F., Heusinkveld, H. J., Zwart, E. P., Hodemaekers, H. M., Rorije, E., de Knecht, J. | Toxicology and Applied Pharmacology 2020; 407:115249 |
| Distinguishing mode of action of compounds inducing craniofacial malformations in zebrafish embryos to support dose-response modeling in combined exposures. | Heusinkveld, H. J., Schoonen, W. G., Hodemaekers, H. M., Nugraha, A., Sirks, J. J., Veenma, V., Sujan, C., Pennings, J. L. A., Wackers, P. F., Palazzolo, L., Eberini, I., Rorije, E., & van der Ven, L. T. M. | Reproductive Toxicology 2020; 96:114-27 |
| An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing | Braakhuis, H. M., He, R., Vandebriel, R. J., Gremmer, E. R., Zwart, E., Vermeulen, J. P., Fokkens, P., Boere, J., Gosens, I., & Cassee, F. R. | Journal of visualized experiments : JoVE 2020; |
| The virtual human in chemical safety assessment | Piersma, A. H., van Benthem, J., Ezendam, J., Staal, Y. C. M., Kienhuis, A. S. | Current Opinion in Toxicology 2019; 15:26-32 |
| Design and validation of an ontology-driven animal-free testing strategy for developmental neurotoxicity testing. Toxicology and applied pharmacology | Hessel, E. V. S., Staal, Y. C. M., & Piersma, A. H. | Toxicology and Applied Pharmacology 2018; 354:136-52 |
| Rethinking developmental toxicity testing: Evolution or revolution? | Scialli, A. R., Daston, G., Chen, C., Coder, P. S., Euling, S. Y., Foreman, J., Hoberman, A. M., Hui, J., Knudsen, T., Makris, S. L., Morford, L., Piersma, A. H., Stanislaus, D., & Thompson, K. E. | Birth defects research 2018; 119(10):480-850 |