In order to study the incidence of CDI in endemic situation, a new sentinel surveillance started in May 2009. Nineteen hospitals participate in this surveillance and introduced a continuous monitoring of CDI. This is the second annual report that provides an overview of the two types of surveillance conducted in the Netherlands. The previous report showed that the incidence in the Netherlands is stable at 15 per 10,000 admissions and the most frequently found types are 001, 078 and 014. In this annual report we describe the situation in the Netherlands between May 1st 2010 and May 1st 2011.