Abstract

The Laboratory of Carcinogenesis and Mutagenesis (LCM) operates a programme on Granuloma Pouch Assay. This technique is used to determine various genotoxic effects induced by radiations and for chemicals in a mesenchymal tissue (subcutaneous fibroblasts) of the rat. In the framework of this assay test experiments were performed in which 6 rats were irradiated with X-rays. The group of 6 rats was divided into three subgroups of 2 animals each, coded 1 (dose=2Gy), 2 (dose=6GY) and 3 (dose=10Gy). The purpose of these preliminary tests is to check wether the applied doses will meet further requirements for assessing the biological endpoints, the capacity involved and the experimental setup.