Publicaties van Aldert Piersma, senior onderzoeker reproductietoxicologie bij het RIVMRijksinstituut voor Volksgezondheid en Milieu.

  1. Brockbank KGM, Piersma AH, van Peer CMJ. The regional distribution of fibroblastic progenitors in murine femoral marrow revisited. Biomedecine 36: 369 371, 1982.

  2. Piersma AH, Ploemacher RE, Brockbank KGM. Transplantation of bone marrow fibroblastoid stromal cells in mice via the intravenous route. Brit.J.Haemat. 54: 285 290, 1983.

  3. Piersma AH, Ploemacher RE, Brockbank KGM. Radiation damage to femoral hemopoietic stroma measured by implant regeneration and quantitation of fibroblastic progenitors. Exp.Hemat. 11: 844 890, 1983.

  4. van Loveren H, de Groot JW, Koten JW, Piersma AH, de Weger RA, den Otter W. A macrophage factor enhancing the systemic antitumour effect of T lymphocytes. Immunobiology 166: 118 130, 1984.

  5. Piersma AH, Brockbank KGM, Ploemacher RE. Regulation of in vitro myelopoiesis by a hemopoietic stromal cell line. Exp.Hemat. 12: 617 623, 1984.

  6. Ploemacher RE, Piersma AH, Brockbank KGM. The nature and function of granulopoietic microenvironments. Blood Cells 10: 341 367, 1984.

  7. Piersma AH, Brockbank KGM, Ploemacher RE, van Vliet PWpartnerwaarschuwing, van Peer CMJ, Visser WJ. Characterization of fibroblastic stromal cells from murine bone marrow. Exp.Hemat. 13: 237 243, 1985.

  8. Brockbank KGM, Piersma AH, Ploemacher RE, Voerman J. Stromal cells (CFU F) in normal and genetically anemic mouse strains. Acta Hemat. 74: 75 80, 1985.

  9. Piersma AH, Ploemacher RE, Brockbank KGM. Recovery of fibroblastoid cells in the hemopoietic stroma after total body irradiation and bone marrow transplantation. Transplantation 40: 198 201, 1985.

  10. Piersma AH, Ploemacher RE, Brockbank KGM, Nikkels PGJ, Ottenheim CPECarbapenamse-producerende enterobacteriaceae. Migration of fibroblastoid stromal cells in murine blood. Cell Tissue Kinetics 18: 589 595, 1985.

  11. Piersma AH, Ploemacher RE, Brockbank KGM. Monoclonal antibodies directed against fibroblastic stromal cells in murine hemopoietic organs. In: Exp. Hemat. Today: 50 54, 1985.

  12. Piersma AH. The nature and function of fibroblastic reticular cells in the hemopoietic stroma. Thesis, Erasmus University Rotterdam, 1985.

  13. Brockbank KGM, de Jong JP, Piersma AH, Voerman JSA. Hemopoiesis on purified bone marrow derived reticular fibroblasts in vitro. Exp. Hemat. 14: 386 394, 1986.

  14. De Jong JP, Nikkels PGJ, Piersma AH, Ploemacher RE. Erythropoiesis and macrophage subsets in medullary and extramedullary sites. In: Molecular and cellular aspects of erythropoiesis and quantitation of erythropoietin. Proc. NATO Advanced Research Workshop, Bad Windsheim, FRG, August 31   September 4, 1986.

  15. Piersma AH, Willemse A, van den Brink CEConformité Européenne, de Laat SW, Mummery CLchloride. Mesodermal differentiation of embryonal carcinoma cells in coculture with visceral endoderm cell lines. In: Cell to Cell Signals in Mammalian Development, NATO ASI Series Vol. H 26: 247 260, 1988.

  16. Mulder EJH, Brader LJ, Verhoef A, Piersma AH, Visser GHA, Peters PWJ. Whole rat embryo culture in serum of insulin dependent (type 1) diabetic women. Toxicology In Vitro 3: 221 226, 1989.

  17. Peters PWJ, Piersma AH. In vitro embryotoxicity and teratogenicity studies. Toxicology in vitro 4: 570 576, 1990.

  18. Piersma AH, Verhoef A, Peters PWJ. Effects of low sodium concentration on the development of postimplantation rat embryos in culture and on their sensitivity to anticonvulsants. Toxicology in Vitro 5:71 75, 1991.

  19. Van den Eijnden van Raaij AJM, van Achterberg TAE, van der Kruijssen CMM, Piersma AH, Huylebroeck D, de Laat SW, Mummery CL. Differentiation of aggregated murine P19 embryonal carcinoma cells is induced by a novel visceral endoderm specific FGF like factor and inhibited by activin A. Mechanisms of Development 33: 157 166, 1991.

  20. Mummery CL, van Achterberg TAE, van den Eijnden van Raaij AJM, van Haaster L, Willemse A, de Laat SW, Piersma AH. Visceral endoderm like cell lines induce differentiation of murine P19 embryonal carcinoma cells. Differentiation 46:51 60, 1991.

  21. Van Maele Fabry G, Picard JJ, Attenon P, Berthet P, Delhaise F, Govers MJAP, Peters PWJ, Piersma AH, Schmid BPBritse Farmacopee, Stadler J, Verhoef A, Verseil C. Interlaboratory evaluation of three culture media for postimplantation rodent embryos. Reproductive Toxicology 5:417 426, 1991.

  1. Piersma AH, van Aerts LAGJM, Verhoef A, Garbis Berkvens JMJoint meeting, Robinson JE, Copius Peereboom Stegeman JHJ, Peters PWJ. Biotransformation of cyclophosphamide in post implantation rat embryo culture using maternal hepatocytes in coculture. Pharmacology and Toxicology 69: 47 51, 1991.

  2. Verhoef A, Piersma AH. Teratologie en reproduktietoxicologie. Biotechniek 32:21-23, 1993.

  3. Piersma AH, Roelen B, Roest P, Haakmat-Hoesenie AS, Van Achterberg TAE, Mummery CL. Cadmium-induced inhibition of proliferation and differentiation of embryonal carcinoma cells in culture and mechanistic aspects of protection by zinc. Teratology 48: 335-341, 1993.

  4. Piersma AH, Haakmat AS, Hagenaars AM. In vitro assays for the developmental toxicity of xenobiotic compounds using differentiating embryonal carcinoma cells in culture. Toxicology in vitro, 7: 615-621, 1993.

  5. Piersma AH. Whole embryo culture and toxicity testing. Toxicology in vitro 7: 763-768, 1993.

  6. Verhoef A, Piersma AH. Nitrofen-geïnduceerde hernia diaphragmatica en longhypoplasie in de rat: een diermodel. Biotechniek 32: 42-44, 1993.

  7. Hessel EM, Verhoef A, Van Loveren H, Piersma AH. Minimal immunologic changes in structurally malformed rats after prenatal exposure to cyclophosphamide. Toxicology 92: 287-299, 1994.

  8. Van der Zee DC, De Heer E, Piersma AH, Vermeij-Keers Chr. Ultrastructural alterations caused by immunological reactions after intracardiac injection of allogeneic antibodies against blood group antigens: An experimental study using the in vitro whole-rat embryo culture. Teratology 52: 57-70, 1995.

  9. Piersma AH, Attenon P, Bechter R, Govers MJAP, Krafft N, Schmid BP, Stadler J, Verhoef A, Verseil C. Interlaboratory evaluation of embryotoxicity in the postimplantation rat embryo culture. Reproductive Toxicology 9: 275-280, 1995.

  10. Piersma AH, Verhoef A, Dortant PM. Evaluation of the OECDOrganisation for Economic Co-operation and Development 421 reproductive toxicity test protocol using butyl benzyl phthalate. Toxicology 99: 191-197, 1995.

  11. Piersma AH, Verhoef A, Dortant PM. Evaluation of the OECD 421 reproductive toxicity test protocol using 1-(butylcarbamoyl)-2-benzimidazolecarbamate (Benomyl). Teratogenesis, Carcinogenesis, and Mutagenesis 15: 93-100, 1995.

  12. Piersma AH, Bechter R, Krafft N, Schmid BP, Stadler J, Verhoef A, Verseil C, Zijlstra J. Interlaboratory evaluation of five pairs of (non-)teratogens in post-implantation rat embryo culture. Alternatives To Laboratory Animals, 24: 201-209, 1996.

  13. Piersma AH, Bode W, Verhoef A, Olling M. Teratogenicity of a single oral dose of retinyl palmitate in the rat and the role of dietary vitamin A status. Pharmacology and Toxicology 79: 131-135, 1996.

  14. Piersma AH. Hormoonontregelaars. Chemische feitelijkheden 146: 1-11, maart 1998.

  15. Piersma AH, Verhoef A, Opperhuizen A, Klaassen R, van Eijkeren J, Olling M. Embryotoxicity of carbamazepine in rat postimplantation embryo culture after in vitro exposure via three different routes. Reproductive Toxicology 12(2): 161-168, 1998.

  16. Scholz G, Pohl I, Seiler A, Bremer S, Brown NA, Piersma AH, Holzhütter HG Spielmann H. Results of the first phase of the ECVAM project “prevalidation and validation of three in vitro embryotoxicity tests”. (in german) ALTEX 15, 3-8, 1998.

  17. Piersma AH. Chemische stoffen, voortplanting en nageslacht. Chemische feitelijkheden 167: 1-15, augustus 1999.

  18. Genschow E, Scholz G, Brown NA, Piersma AH, Brady M, Clemann N, Huuskonen H, Paillard F, Bremer S, Spielmann H. Development of prediction models for three in vitro embryotoxicity tests which are evaluated in an ECVAM validation study. (in german) ALTEX 16, 73-83, 1999.

  19. Piersma AH, Verhoef A, Elvers LH, Wester PW. Toxicity of compounds with endocrine activity in the OECD 421 reproductive toxicity screening test. In: Endocrine disrupting compounds: wildlife and human health risks. Symposium Proc. Eds. Vethaak AD, Rijs GBJ, vanderBurg B, Brouwer A, pp 58-60, 2000.

  20. Genschow E, Scholz G, Brown NA, Piersma AH, Brady M, Clemann N, Huuskonen H, Paillard F, Bremer S, Becker K, Spielmann H. Development of prediction models for three in vitro embryotoxicity tests in an ECVAM validation study. In Vitro & Molecular Toxicol. 13, 51-65, 2000.

  21. Beekhuijzen MEW, Verhoef A, Klaassen R, Rompelberg CJM, Piersma AH. Comparison of in vitro and in vivo developmental toxicity and pharmacokinetics of phenytoin in the rat. Pharmacol. and Toxicol. 87: 96-102, 2000.

  22. Piersma AH, Verhoef A, te Biesebeek JDCreutzfeldt-Jakob, Pieters MN, Slob W. Developmental toxicity of butylbenzylphthalate in the rat using a multiple dose study design. Reprod. Toxicol. 14: 417-425, 2000.

  23. Piersma AH. The EUEuropean Union Guideline on Classification and Labelling of Substances Toxic to reproduction (Council Directive 67/548/EEC on Dangerous Substances). In: Current epidemilogical evidence versus experimental data on reproductive and developmental toxicity of pesticides. Procedings of an Euroworkshop, Ed. T Vergieva and F. Kaloyanova-Simeonova, ISBN 954-90924-1-0, pp. 245-253, 2001.

  24. Spielmann H, Genschow E, Scholz G, Brown NA, Piersma AH, Brady M, Clemann N, Huuskonen H, Paillard F, Bremer S, Becker K. Preliminary results of the ECVAM validation study on three in vitro embryotoxicity tests. ATLA 29: 301-303, 2001.

  25. Hollander G de, Piersma AH. Hormonen in water. In: Gevaar van water, water in gevaar. Ed. van Everdingen JJE, Goudsmit J. Belvédère/Medidact, Overveen/Alphen aan den Rijn, The Netherlands, pp. 101-115, 2001.

  26. Genschow E, Spielmann H, Scholz G, Seiler A, Brown N, Piersma A, Brady M, Clemann N, Huuskonen H, Paillard F, Bremer S, Becker K. The ECVAM international validation study on in vitro embryotoxicity tests: results of the definitive phase and evaluation of prediction models. Altern Lab Anim. 30(2):151-176, 2002.

  27. Piersma AH, Verhoef A, Sweep CGJ, de Jong WH, van Loveren H. Developmental toxicity but no immunotoxicity in the rat after prenatal exposure to diethylstilbestrol. Toxicology 174(3): 173-181, 2002.

  28. H. van Loveren, J. Vosvluchtige organische stoffen, E. Putman, A.H. Piersma. Immunotoxicological consequences of perinatal chemical exposures: a plea for inclusion of immune parameters in reproduction studies. Toxicology 185/3 pp 185 – 191, 2002.

  1. Siemelink M, Verhoef A, Dormans JAMAJournal of the American Medical Association, Span PN, Piersma AH. Dietary fatty acid composition during pregnancy and lactation in the rat programs growth and glucose metabolism in the offspring. Diabetologia 45: 1397-1403, 2002.

  2. Dayan A, Brown N, Faustman E, McGready R, Paulus G, Piersma AH, Sullivan F. Assessment of the safety of artemisinin compounds in pregnancy. WHO/CDS/MAL/2003.1094. WHO/RBMRisk Based Maintainance/TDR/Artemisinin/03.1, 2003.

  3. M. Moerbeek, A.H. Piersma, W. Slob. A comparison of three methods for calculating confidence intervals for the benchmark dose. Risk Analysis 24(1): 31-40, 2004.

  4. Kroes R, Renwick AG, Cheeseman M, Kleiner J, Mangelsdorf I, Piersma A, Schilter B, Schlatter J, van Schothorst F, Vos JG, Wurtzen G. Structure-based thresholds of toxicological concern (TTC): guidance for application to substances present at low levels in the diet. Food Chem Toxicol. 2004 Jan; 42(1): 65-83.

  5. Piersma AH. Validation of alternative methods for developmental toxicity testing. Toxicology Letters 2004 149: 147-153, 2004.

  6. Loveren H van, Piersma AH. Immunological consequences of perinatal chemical exposures. Toxicol. Lett. 149: 141-145, 2004.

  7. Piersma AH, Genschow E, Verhoef A, Spanjersberg MQI, Brown NA, Brady M, Burns A, Clemann N, Seiler A, Spielmann H. Validation of the postimplantation rat whole embryo culture test in the International ECVAM Validation study on three in vitro embryotoxicity tests. ATLA 32: 275-307, 2004.

  8. Spielmann H, Genschow E, Brown NA, Piersma AH, Verhoef A, Spanjersberg MQI, Huuskonen H, Paillard F, Seiler A. Validation of the rat limb bud micromass test in the international ECVAM validation study on three in vitro embryotoxicity tests. ATLA 32: 245-274, 2004.

  9. Luijten M, Ronfeldt Thomson A, van den Berg JAH, Wester PW, verhoef A, Nagelkerke NJD, Adlercreutz H, van Kranen HJ, Piersma AH, Sorensen IK, Rao GN, van Kreijl CFCystic Fibrosis. Effects of soy-derived isoflavones and a high-fat diet on spontaneous mammary tumor development in Tg.NK (MMTV/c-neu) mice. Nutrition and cancer 50(1): 46-54, 2004.

  10. Slob W, Moerbeek M, Rauniomaa E, Piersma AH. A Statistical Evaluation of Toxicity Study Designs for the Estimation of the Benchmark Dose in Continuous Endpoints. Toxicological Sciences 84(1):167-185, 2005.

  11. Piersma AH. Editorial. On the Occasion of the Special Issue on the 33d Annual Conference of the European Teratology Society. Reproductive Toxicology 20(3): 287, 2005.

  12. Piersma AH. Alternatives to animal testing in developmental toxicology. Invited Review Toxicology & Pharmacology 98: 427-431, 2006.

  13. Silke Germer; Piersma Aldert H, DrDoctor .; Leo van der Ven, Dr.; Andreas Kamyschnikow; Yvonne Fery; Hans-Joachim Schmitz, Dr.; D. Schrenk Subacute effects of the brominated flame retardants hexabromocyclododecane and tetrabromobisphenol A on hepatic cytochrome P450 levels in rats. Toxicology 218(2-3):229-36, 2006.

  14. Germer, S., Fery, Y., Kamyschnikow, A., Piersma, A.H., Van der Ven, L.T.M., Schrenk, D. Induction of cytochrome p450 enzymes in rat liver and rat primary hepatocytes by polybrominated diphenyl ethers. Toxicol.Lett. 164, S159-S160, 2006.

  15. Fabjan, E., Hulzebos, E., Mennes, W. and Piersma, A.H. A category approach for reproductive effects of phthalates. Crit. RevRevalidatiecentra. Toxicol. 36:695-726, 2006.

  16. Ven LTM van der, Verhoef A, van de Kuil T, Slob W, leonards PEG, Visser TJ, Hamers T, Herlin M, Hakansson H, Olausson H, Piersma A.H., Vos JG. A 28 day oral toxicity study enhanced to detect endocrine effects of hexabromocyclododecane in wistar rats. Tox.Sci. 94(2), 281-292, 2006.

  17. Spielmann H, Seiler A, Bremer S, Hareng L, Hartung T, Ahr H, Faustman E, Hass U, Moffat GJ, Nau H, Vanparys P, Piersma AH, Riego Sintes J, Stuart J. The practical application of three validated in vitro embryotoxicity tests. ATLA 34: 527-538, 2006.

  18. Luijten M, Verhoef A, Dormans J, Beems R, Cremers J, Nagelkerke N, Adlercreutz H, Penalvo J and Piersma AH. Modulation of Mammary Tumor Development In Tg.NK (MMTV/c-neu) Mice by Dietary Fatty Acids and Life Stage-Specific Exposure to Phytoestrogens. Reproductive Toxicology 23(3), 407-413, 2007.

  19. van Meeuwen, J.A., Korthagen, N., de Jong, P.C., Piersma, A.H., van den Berg, M., (Anti)estrogenic effects of phytochemicals on human primary mammary fibroblasts, MCF-7 cells and their co-culture, Toxicology and Applied Pharmacology 2007 221(3):372-83.

  20. van Meeuwen, J.A., van den Berg, M., Sanderson, J.T., Verhoef, A., Piersma, A.H., Estrogenic effects of mixtures of phyto- and synthetic chemicals on uterine growth of prepubertal rats, Toxicology Letters (2007): 170(2):165-76.

  21. van Meeuwen, J.A., W. ter Burg, A.H. Piersma, M. van den Berg and J.T. Sanderson Mixture effects of estrogenic compounds on proliferation and pS2 expression of MCF-7 human breast cancer cells, Food and Chemical Toxicology, 2007, 45 (2007) 2319–2330.

  22. Janer G, Hakkert BC, Piersma AH, Vermeire T, Slob W. A retrospective analysis of the added value of the rat two-generation reproductive toxicity study versus the rat subchronic toxicity study Reproductive Toxicology, 2007, 24: 103-113.

  23. Janer G, Hakkert BC, Slob W, Vermeire T, Piersma AH. A retrospective analysis of the two-generation study: What is the added value of the second generation? Reproductive Toxicology, 2007, 24: 97-102. Prize winning article

  24. Aldert H. Piersma, Gemma Janer, Aart Verhoef, Gerrit Wolterink, Wout Slob. Quantitative extrapolation of in vitro whole embryo culture embryotoxicity data to developmental toxicity in vivo using the Benchmark approach. Toxicological Sciences 101(1), 91–100, 2008.

  25. Leo T.M. van der Ven, Ton van de Kuil, Aart Verhoef, PimPneumokokken Iets Minder E.G. Leonards, Wout Slob, Rocıo F. Canton, Silke Germer, Timo Hamers, Theo J. Visser, Sabina Litens, Helen Hakansson, Yvonne Fery, Dieter Schrenk, Martin van den Berg, Aldert H. Piersma, Josephus G. Vos. A 28-day oral dose toxicity study enhanced to detect endocrine effects of a purified technical pentabromodiphenyl ether (pentaBDE) mixture in Wistar rats. Toxicology 245, 2008, 109–122.

  1. Leo T.M. Van der Ven, Ton Van de Kuil, Aart Verhoef, Cynthia M. Verwer, Hellmuth Lilienthal, Pim E.G. Leonards, Ute M.D. Schauer Rocıo F. Canton, Sabina Litens, Frank H. De Jong, Theo J. Visser, Wolfgang Dekant, Natalia Stern, Helen Hakansson, Wout Slob, Martin Van den Berg, Josephus G. Vos, Aldert H. Piersma. Endocrine effects of tetrabromobisphenol-A (TBBPA) in Wistar rats as tested in a one-generation reproduction study and a subacute toxicity study Toxicology 2008 Mar 12;245(1-2):76-89.

  2. Hellmuth Lilienthal, Cynthia Verwer, Leo van der Ven, Aldert Piersma, Josephus Vos. Neurobehavioral Effects of Tetrabromobisphenol A (TBBPA) in Rats after Pre- and Postnatal Exposure, Toxicology 246, 2008, 45–54.

  3. Anne-Maj Samuelsson, Phillippa A. Matthews, Michael R. Christie, Josie M. McConnell, Eugene H.J.M. Jansen, Aldert H. Piersma, Susan E.Ozanne, Denise Fernandez Twinn, Claude Remacle, Athea Rowlerson, Lucilla Poston, & Paul D. Taylor. Diet-induced obesity in female mice leads to offspring hyperphagia, adiposity, hypertension, and insulin resistance. A novel model of developmental programming Hypertension. 2008; 51(2):383-92.

  4. Janer G, Slob W, Hakkert B, Vermeire T, Piersma AH. A retrospective analysis of developmental toxicity studies: what is the added value of the rabbit as an additional species? Regulatory Toxicology and Pharmacology, 50(2):206-17, 2008.

  5. Leo T.M. Van der Ven, Ton van de Kuil, Pim E.G. Leonards,Wout Slob, Rocıo F. Canton, Silke Germer, Theo J. Visser, Sabina Litens, Helen Ha˚ kansson, Dieter Schrenk, Martin van den Berg, Aldert H. Piersma, Josephus G. Vos, Antoon Opperhuizen. A 28-day oral dose toxicity study in Wistar rats enhanced to detect endocrine effects of decabromodiphenyl ether (decaBDE) Toxicology Letters 179 (2008) 6–14.

  6. J.A. van Meeuwen, O. van Son, A.H. Piersma, P.C. de Jong and M. van den Berg. Aromatase inhibiting and combined estrogenic effects of parabens and estrogenic effects of other additives in cosmetics. Toxicology and Applied Pharmacology, 230(3): 269-276, 2008.

  7. J.A. van Meeuwen, S. Nijmeijer, T. Mutarapat, S. Ruchirawat, P.C. de Jong, A.H. Piersma and M. van den Berg. Aromatase inhibition by synthetic lactones and flavonoids in human placental microsomes and breast fibroblasts – A comparative study. Toxicology and Applied Pharmacology, 228(3):269-76, 2008.

  8. Luijten M, Verhoef A, Westerman A, Piersma AH. Application of a metabolizing system as an adjunct to the rat whole embryo culture.Toxicology in vitro, 2008 Aug;22(5):1332-6.

  9. Janer G, Hakkert BC, Slob W, Vermeire T, Piersma AH. A retrospective analysis of the two-generation study, author response to letter to the editor. Reproductive Toxicology 25 (2008) 406–407.

  10. Cantón RF, Peijnenburg AA, Hoogenboom RLrichtlijn, Piersma AH, van der Ven LT, van den Berg M, Heneweer M. Subacute effects of hexabromocyclododecane (HBCD) on hepatic gene expression profiles in rats. Toxicol Appl Pharmacol. 2008 Sep 1;231(2):267-72.

  11. Robert Chapin, Karen Augustine-Rauch, Bruce Beyer, George Daston, Richard Finnell, Thomas Flynn, Sidney Hunter, Phillip Mirkes, K. Sue O’Shea, Aldert Piersma, David Sandler, Philippe Vanparys, Geneviève Van Maele-Fabry The State of the Art in Developmental Toxicity Screening Methods, and The Way Forward. Birth Defects Res B Dev Reprod Toxicol. 2008 Aug;83(4):446-56.

  12. Janer G, Verhoef A, Gilsing HD, Piersma AH Use of the rat postimplantation embryo culture to assess the embryotoxic potency within a chemical category and to identify toxic metabolites. Toxicology in Vitro 22 (2008) 1797–1805.

  13. Leo T.M. van der Ven, Wout Slob, Aldert H. Piersma, Pim E.G. Leonards, Timo Hamers, Anna Sandholm, Martin Van den Berg, Antoon Opperhuizen. More on the toxicity of decabromodiphenyl ether—Response to Hardy et al. (2008). Toxicology Letters 182 (2008) 130–132.

  14. Lilienthal H, van der Ven LT, Piersma AH, Vos JG. Effects of the brominated flame retardant hexabromocyclododecane (HBCD) on dopamine-dependent behavior and brainstem auditory evoked potentials in a one-generation reproduction study in Wistar rats. Toxicol Lett. 2009 Feb 25;185(1):63-72

  15. Peter M.J. Bos, Polly E. Boon, Hilko van der Voet, Gemma Janer, Aldert H. Piersma, Beat Brüschweiler, Elsa Nielsen, Wout Slob. A semi-quantitative model for risk appreciation and risk weighing, Food Chem. Tox. 2009, 47(12):2941-50

  16. H.-D. Gilsing, H. Faltz, G. Janer, A. H. Piersma "Useful strategies for the synthesis of oxidative metabolites of Di-n-butyl phthalate (DBP) and Bis (2-ethylhexyl) phthalate (DEHPdiethylhexylftalaat) containing a short-chain carboxylic functional group" Manuscript in preparation.

  17. Dorien A.M. van Dartel, Jeroen L.A. Pennings, Peter J.M. Hendriksen, Frederik J. van Schooten, Aldert H. Piersma. Early gene expression changes during embryonic stem cell differentiation into cardiomyocytes and its modulation by monobutylphthalate. Reproductive Toxicology 27: 93-102, 2009.

  18. Leo T.M. van der Ven, Ton van de Kuil, Pim E.G. Leonards,Wout Slob, Hellmuth Lilienthal, Sabina Litens, Maria Herlin, Helen Håkansson, Rocío F. Cantón, Martin van den Berg, Theo J. Visser, Henk van Loveren, Josephus G. Vos, Aldert H. Piersma. Endocrine effects of hexabromocyclododecane (HBCD) in a one-generation reproduction study in Wistar rats. Toxicology Letters 185 (2009) 51–62.

  19. Hellmuth Lilienthal, Leo van der Ven, Alfons Hack, Astrid Roth-Härer, Aldert Piersma, and Joseph Vos. Neurobehavioral Effects in Relation to Endocrine Alterations Caused by Exposure to Brominated Flame Retardants in Rats—Comparison to Polychlorinated Biphenyls. Human and Ecological Risk Assessment, 15: 76–86, 2009. Taylor & Francis Group, LLC, ISSN: 1080-7039 print / 1549-7680 online

  20. Dorien A.M. van Dartel, Nicole J.L. Zeijen, Liset J.J. de la Fonteyne, Frederik J. van Schooten, Aldert H. Piersma. Disentangling cellular proliferation and differentiation in the Embryonic Stem cell Test, and its impact on the experimental protocol. Reproductive Toxicology, 28 (2009) 254–261.

  21. Zhi-Chao Dang, Emiel Rorije, Thijs Hogen Esch, André Muller, Betty C. Hakkert, Aldert H. Piersma. Retrospective analysis of relative parameter sensitivity in multi-generation reproductive toxicity studies. Reproductive Toxicology 28 (2009) 196–202.

  1. Esther de Jong, Jochem Louisse, Miriam Verwei, Bas J. Blaauboer, HanHogeschool van Arnhem en Nijmegen van de Sandt, Ruud A. Woutersen, Ivonne M.C.M. Rietjens, Aldert H. Piersma. Relative developmental toxicity of glycol ether alkoxy acetic acid metabolites in the embryonic stem cell test as compared to the in vivo potency of their parent compounds. Tox. Sci. 110(1), 117–124 (2009).

  2. van der Ven LT, Slob W, Piersma AH, Opperhuizen A. The benchmark approach is the preferred method to describe the toxicology of Tetrabromobisphenol A (TBBPA)-Response to Banasik et al. Toxicology. 2009 Jun 16;260(1-3):153-4.

  3. Lilienthal H, Slob W, van der Ven LT, Piersma AH. Measurement and evaluation of neurobehavioral effects induced by tetrabromobisphenol A (TBBPA)-Response to. Toxicology. 2009 Jun 16;260(1-3):158-61.

  4. Park MV, Annema W, Salvati A, Lesniak A, Elsaesser A, Barnes C, McKerr G, Howard CV, Lynch I, Dawson KA, Piersma AH, Jong WH de. In vitro developmental toxicity test detects inhibition of stem cell differentiation by silica nanoparticles. Toxicol Appl Pharmacol. 2009, 240(1):108-16.

  5. Philip Marx-Stoelting, Els Adriaens, Hans-Jürgen Ahr, Susanne Bremer, Bernward Garthoff, Heinz-Peter Gelbke, Aldert Piersma, Cristian Pellizzer, Ulrike Reuter, Vera Rogiers, Barbara Schenk, Silke Schwengberg, Andrea Seiler, Horst Spielmann, Margino Steemans, Donald B. Stedman, Philippe Vanparys, Joan Albert Vericat, Miriam Verwei, Femke van de Water, Marc Weimer and Michael Schwarz. A Review of the Implementation of the Embryonic Stem Cell Test (EST) The report and recommendations of an ECVAM/ReProTect Workshop. ATLA 37, 313–328, 2009.

  6. Bos PM, Boon PE, van der Voet H, Janer G, Piersma AH, Brüschweiler BJ, Nielsen E, Slob W. A semi-quantitative model for risk appreciation and risk weighing. Food Chem Toxicol. 2009 Dec;47(12):2941-50.

  7. Tonk, ECM, Piersma AH, van Loveren H. Reproductive and Developmental Immunology. Comparative Toxicology 2nd Ed., Elsevier, Ed. Charlene McQueen; Chapter, Chapter 5.13, p249-269, 2010.

  8. Piersma AH, van Dartel DAM, van der Ven LPM. Alternative Methods in Reproductive Toxicology. Book chapter, Comparative Toxicology 2nd Ed., Elsevier, Ed. Charlene McQueen; Chapter, Chapter 12.20 p 293-305, 2010.

  9. Dorien A.M. van Dartel, Jeroen L.A. Pennings, Frederik J. van Schooten, Aldert H. Piersma. Transcriptomics-based Identification of Developmental Toxicants through their Interference with Cardiomyocyte Differentiation of Embryonic Stem Cells. Toxicology and Applied Pharmacology 243: 420–428, 2010.

  10. Barbara Schenk, Marc Weimer, Susanne Bremer, Bart van der Burg, Rita Cortvrindt, Alexius Freyberger, Giovanna Lazzari, Cristian Pellizzer, Aldert Piersma, Wolfgang Schäfer, Andrea Seiler, Hilda Witters, Michael Schwarz. The ReProtect Feasibility Study, Reproductive Toxicology 30: 200-218, 2010.

  11. Ahmed M. Osman, Dorien A.M. van Dartel, Edwin Zwart, Marco Blokland, Jeroen L.A. Pennings, Aldert H. Piersma. Proteome profiling of mouse embryonic stem cells to monitor cell differentiation and its modulation by monobutyl phthalate. Reproductive Toxicology 30 (2010) 322–332, 2010.

  12. Theunissen PTpertussis-toxine, Schulpen SHW, van Dartel DAM, Hermsen SAB, van Schooten FJ, Piersma AH. An abbreviated protocol for multilineage neural differentiation of murine embryonic stem cells and its perturbation by methylmercury. Reproductive Toxicology 2010 Jul;29(4):383-92.

  13. Mirjam Luijten, Vincent van Beelen, Aart Verhoef, Marc Renkens, Marcel van Herwijnen, Anja Westerman, Frederik-Jan van Schooten, Jeroen Pennings, Aldert H. Piersma. Transcriptomics analysis of retinoic acid embryotoxicity in rat postimplantation whole embryo culture. Reproductive Toxicology 30, 333-340, 2010.

  14. Aldert Piersma. Editorial. Reproductive Toxicology Volume 30(1), Page 1, 2010.

  15. Esther de Jong, Wout Slob, Aldert H. Piersma. Application of the benchmark approach in the correlation of in vitro and in vivo data in developmental toxicity. In: Methods in Bioengineering: Alternative Technologies to Animal Testing, ed. TimTweede Immunisatie MenC Maguire & Eric Novik. Artech House, Boston, London, Chapter 9, pp. 159-169, 2010.

  16. Dorien A.M. van Dartel, Jeroen L.A. Pennings, Liset J.J. de la Fonteyne, Marcel H. van Herwijnen, Joost H. van Delft, Frederik J. van Schooten, Aldert H. Piersma, Monitoring Developmental Toxicity in the Embryonic Stem Cell Test Using Differential Gene Expression of Differentiation-related Genes. Toxicol Sci. 2010 Jul;116(1):130-9.

  17. ElisaEnzyme-Linked Immunosorbent assay CM Tonk, Didima MG de Groot, André H Penninks, Ine DH Waalkens-Berendsen, André PM Wolterbeek, Wout Slob, Aldert H Piersma, Henk van Loveren. Developmental Immunotoxicity Of Methylmercury: The Relative Sensitivity Of Developmental And Immune Parameters. Toxicological Sciences 117(2):325-35. 2010.

  18. Jochem Louisse, Esther de Jong, Johannes J.M. van de Sandt, Bas J. Blaauboer, Ruud A. Woutersen, Aldert H. Piersma, Ivonne M.C.M. Rietjens, Miriam Verwei. The use of in vitro toxicity data and physiologically based kinetic modeling to predict dose-response curves for in vivo developmental toxicity of glycol ethers in rat and man. Toxicol Sci. 118(2):470-484, 2010. prize winning article

  19. George P. Daston, Robert E Chapin, Anthony R. Scialli, Aldert H. Piersma, Edward W. Carney, John M. Rogers, Jan M. Friedman. A Different Approach to Validating Screening Assays for Developmental Toxicity. Birth Defects Research (Part B) 83:1–5, 2010.

  20. Joshua F. Robinson, Vincent A. van Beelen, Aart Verhoef, Marc F.J. Renkens, Mirjam Luijten, Marcel H.M. van Herwijnen, Anja Westerman, Jeroen L.A. Pennings, Aldert H. Piersma. Embryotoxicant-specific transcriptomics responses in rat postimplantation whole embryo culture. Toxicological Sciences 118(2):675-685, 2010.

  21. Dorien A.M. van Dartel, Jeroen L.A. Pennings, Liset J.J. de la Fonteyne, Karen J.J. Brauers, Sandra Claessen, Joost H. van Delft, Jos C.S. Kleinjans, Aldert H. Piersma. Evaluation of Developmental Toxicant Identification Using Gene Expression Profiling in Embryonic Stem Cell Differentiation Cultures. Toxicol Sci. 119(1):126-34. 2011.

  22. Adler S, Basketter D, Creton S, Pelkonen O, van Benthem J, Zuang V, Andersen KE, Angers-Loustau A, Aptula A, Bal-Price A, Benfenati E, Bernauer U, Bessems J, Bois FY, Boobis A, Brandon E, Bremer S, Broschard T, Casati S, Coecke S, Corvi R, Cronin M, Daston G, Dekant W, Felter S, Grignard E, Gundert-Remy U, Heinonen T, Kimber I, Kleinjans J, Komulainen H, Kreiling R, Kreysa J, Leite SB, Loizou G, Maxwell G, Mazzatorta P, Munn S, Pfuhler S, Phrakonkham P, Piersma A, Poth A, Prieto P, Repetto G, Rogiers V, Schoeters G, Schwarz M, Serafimova R, Tähti H, Testai E, van Delft J, van Loveren H, Vinken M, Worth A, Zaldivar JM. Alternative (non-animal) methods for cosmetics testing: current status and future prospects-2010. Arch Toxicol. 2011 May;85(5):367-485.

  23. Pennings JL, van Dartel DA, Robinson JF, Pronk TE, Piersma AH. Gene set assembly for quantitative prediction of developmental toxicity in the embryonic stem cell test. Toxicology 2011: 284(1-3):63-71.

  24. Pennings J, van Dartel D, Pronk T, Hendriksen P, Piersma A. Identification by gene co-regulation mapping of novel genes involved in embryonic stem cell differentiation. Stem Cells Dev. 20(1): 115-126, 2011.

  25. Dorien A.M. van Dartel, Jeroen L.A. Pennings, Liset J.J. de la Fonteyne, Karen J.J. Brauers, Sandra Claessen, Joost H. van Delft, Jos C.S. Kleinjans, Aldert H. Piersma. Concentration-dependent Gene Expression responses to Flusilazole in Embryonic Stem Cell Differentiation Cultures. Toxicology and Applied Pharmacology 251, 110–118, 2011.

  26. E. de Jong, A. Doedée, M.A. Reis-Fernandes, H. Nau, A.H.Piersma. Potency ranking of valproic acid analogues as to inhibition of cardiac differentiation of embryonic stem cells in comparison to their in vivo embryotoxicity. Reprod. Toxicol. Reprod Toxicol. 2011 May;31(4):375-82.

  27. Sanne A.B. Hermsen, Evert-Jan van den Brandhof, Leo T.M. van der Ven, Aldert H. Piersma. Relative embryotoxicity of two classes of chemicals in a modified zebrafish embryotoxicity test and comparison with their in vivo potencies. Toxicol. In vitro, 25(3):745-53, 2011.

  28. Dorien A.M. van Dartel, Jeroen L.A. Pennings, Joshua F. Robinson, Jos C.S. Kleinjans, Aldert H. Piersma. Discriminating classes of developmental toxicants using gene expression profiling in the embryonic stem cell test. Toxicol Lett. 201(2):143-51, 2011.

  29. Piersma AH, Rorije E, Beekhuijzen ME, Cooper R, Dix DJ, Heinrich-Hirsch B, Martin MT, Mendez E, Muller A, Paparella M, Ramsingh D, Reaves E, Ridgway P, Schenk E, Stachiw L, Ulbrich B, Hakkert BC. Combined retrospective analysis of 498 rat multi-generation reproductive toxicity studies: on the impact of parameters related to F1 mating and F2 offspring. Reprod Toxicol. 31(4), 392-401, 2011.

  30. Piersma AH, Hernandez L, van Benthem J, Muller AJJ, van Leeuwen FXR, Vermeire TG, van Raaij MTM. Reproductive Toxicants have a Threshold of Adversity. Crit Rev Toxicol. 41(6): 545-54, 2011.

  31. Bart van der Burg, E. Dinant Kroese, Aldert H. Piersma. Towards a Pragmatic Alternative Testing Strategy for the Detection of Reproductive Toxicants. Reproductive Toxicology 31(4), 558-561, 2011.

  32. Esther de Jong, Marta Barenys, Sanne AB. Hermsen, Aart Verhoef, Bernadette C. Ossendorp, Jos GM. Bessems, Aldert H. Piersma. Comparison of the mouse Embryonic Stem cell Test, the rat Whole Embryo Culture and the Zebrafish Embryotoxicity Test as alternative methods for developmental toxicity testing of six 1,2,4-triazoles. Toxicol Appl Pharmacol. 253(2):103-11, 2011.

  33. Elisa CM Tonk, Didima MG de Groot, André H Penninks, Ine DH Waalkens-Berendsen, André PM Wolterbeek, Aldert H Piersma, Henk van Loveren. Extended One-generation Reproduction Study in Rats with DOTC – Additional Parameters for Effects on the Immune System. Toxicol Lett. 204(2-3):156-63, 2011.

  34. Sanne A.B. Hermsen, Tessa Pronk, Evert-Jan van den Brandhof, Leo T.M. van der Ven, Aldert H. Piersma. Class specific gene expression changes in the zebrafish embryo after exposure to three glycol ether alkoxy acids and two 1,2,4-triazole antifungals. Reprod. Toxicol. 32(2):245-52, 2011.

  35. Dorien A.M. van Dartel, Aldert H. Piersma. The embryonic stem cell test combined with toxicogenomics as an alternative testing model for the assessment of developmental toxicity. Review, Reproductive Toxicology, Reprod Toxicol. 32(2):235-44, 2011.

  36. Theunissen PT, Pennings J, Robinson JF, Kleinjans JCS, Piersma AH. Time-response evaluation by transcriptomics of methylmercury effects on neural differentiation of murine embryonic stem cells. Toxicol Sci. 122(2):437-47, 2011.

  37. Joshua F. Robinson, Theunissen PT, van Dartel DA, Pennings JL, Faustman EM, Piersma AH.Comparison of MeHg-induced toxicogenomic responses across in vivo and in vitro models used in developmental toxicology. Reprod Toxicol. 2011 Sep;32(2):180-8, 2011.

  38. E. Rorije, A. Muller, M. E.W. Beekhuijzen, U. Hass, B. Heinrich-Hirsch, M. Paparella, E.Schenk, B. Ulbrich, B.C. Hakkert, A.H. Piersma. On the impact of second generation mating and offspring in multi-generation reproductive toxicity studies on Classification and Labelling of substances in Europe. Regul. Toxicol. Pharmacol, 2011 NovNederlandse Orthopaedische Vereniging;61(2):251-60.

  39. Aldert H. Piersma. Embryonic Stem Cell approaches towards biomarkers of embryotoxicity. AXLR8-2 Workshop Report, Berlin, 24 May 2011, ISBN 978-3-9814126-2-8, pp. 244-252.

  40. Elisa Tonk; Aart Verhoef; Liset de la Fonteyne; Ine Waalkens-Berendsen; André Wolterbeek; Henk van Loveren; Aldert Piersma. Developmental immunotoxicity in male rats after juvenile exposure to di-n-octyltin dichloride (DOTC). Reprod Toxicol. 2011 Nov;32(3):341-8.

  41. Peter T. Theunissen, Aldert H. Piersma. Innovative approaches in the embryonic stem cell test (EST). In: Embryonic Stem Cell-Based Novel Alternative Testing Strategies for Drug Development. (http://www.bioscience.org) Managing Editor: Agapios Sachinidis, Frontiers in Bioscience 17, 1965-1975, January 1, 2012.

  42. Theunissen PT, Robinson JF, Pennings JLA, de Jong E, Claessen SMH, Kleinjans JCS Piersma AH. Transcriptomic ­­concentration-response evaluation of Valproic Acid, Cyproconazole and Hexaconazole in the neural embryonic stem cell test (ESTn). Toxicol Sci. 2012 Feb;125(2):430-8.

  43. Joshua F. Robinson, Aart Verhoef, Jeroen L.A. Pennings, Tessa E. Pronk, Aldert H. Piersma. A Comparison between In Vivo and Whole Embryo Culture using Transcriptomics: Time-Dependent Retinoic Acid-induced Teratogenic Response. Toxicol. Sci. 2012, 126: 242-254.

  1. Joshua F. Robinson, Aart Verhoef, Aldert H. Piersma. Transcriptomic Analysis of Neurulation and Early Organogenesis in Rat Embryos: an In Vivo and In Vitro Comparison. Toxicol. Sci. 2012, 126: 255-266.

  2. Ellis-Hutchings, R.G. De Jong, E. Piersma, A.H. Carney, E.W. In vitro screening methods for developmental toxicology. Developmental and Reproductive Toxicology: A Practical Approach. Ronald Hood, Editor. Third edition. 2011 London, Informa Healthcare, chapter 13, pp 411-428. ISBN 9781841848211.

  3. Elisa CM Tonk, Aart Verhoef, Eric Gremmer, Henk van Loveren, Aldert H Piersma. Relative sensitivity of reproductive and immune parameters in juvenile versus adult male rats after exposure to di(2ethyl hexyl) phthalate, Toxicol Appl Pharmacol. 2012 Apr 1;260(1):48-57.

  1. Hermsen SA, Pronk TE, van den Brandhof EJ, van der Ven LT, Piersma AH. Concentration-response analysis of differential gene expression in the zebrafish embryotoxicity test following flusilazole exposure. Toxicol Sci. 2012 May;127(1):303-12.

  2. Judith C. Madden, Mark Hewitt, Katarzyna Przybylak, Rob Vandebriel, Aldert H. Piersma, Mark T. D. Cronin. Strategies for the Optimisation of In Vivo Experiments in Accordance with the 3Rs Philosophy. Regulatory Toxicology and Pharmacology 63 (2012) 140–154

  3. Jan Willem van der Laan, Robert E. Chapin, BertBooster tegen pertussis (kinkhoest) Haenen, Abigail C. Jacobs, Aldert Piersma. Testing strategies for embryo-fetal toxicity of human pharmaceuticals. Animal models vsVerenigde Staten. in vitro approaches. A workshop report. Regul Toxicol Pharmacol. 2012 Mar 16;63(1):115-123.

  4. Joshua F. Robinson, Jeroen L.A. Pennings, Aldert H. Piersma. A Review of Toxicogenomic Approaches in Developmental Toxicity. In: Methods in Molecular Biology, Ed. Craig Harris % Jason M. Hansen; 889: 347-371, 2012.

  5. Theunissen PT, Robinson JF, Pennings JLA, van Herwijnen MH, Kleinjans JCS, Piersma AH. Compound-specific effects of diverse neurodevelopmental toxicants on global gene expression in the neural embryonic stem cell test (ESTn). Toxicol Appl Pharmacol. 2012, 262(3):330-40.

  6. Esther de Jong, Lianne van Beek, Aldert H. Piersma. Osteoblast differentiation of murine embryonic stem cells as a model to study the embryotoxic effect of compounds. Toxicology in vitro 2012, 26(6):970-8.

  7. Jeroen L. A. Pennings, Peter T. Theunissen, and Aldert H. Piersma. An optimized gene set for transcriptomics based neurodevelopmental toxicity prediction in the neural embryonic stem cell test. Toxicology 300 (2012) 158– 167.

  8. Aldert H. Piersma. Animal study protocols and alternative assays for the assessment of reproductive and developmental toxicity. In: Computational Methods for Reproductive and Developmental Toxicology, Ed. D. Mattison, CRCChemical Review Committee Press/Taylor and Francis Group. accepted.

  9. Aldert H. Piersma. Innovations in testing strategies in reproductive toxicology. In: Teratogenicity Testing: Methods and Protocols, Springer Protocols Series, Ed. Paul Barrow, accepted.

  10. Aldert H. Piersma, Elisa C.M. Tonk, Susan L. Makris, Kevin M. Crofton, Rodney R. Dietert, Henk van Loveren. Juvenile toxicity testing protocols for chemicals, Reprod. Toxicol. , accepted.

  11. Joshua F. Robinson, Elisa C.M. Tonk, Aart Verhoef, Aldert H. Piersma. Triazole Induced Concentration-Related Gene Signatures in Rat Whole Embryo Culture. ETSEuropean Teratology Society special issue 2012, accepted.

  12. E. Rorije, F.J.W. van Hienen, Z.C. Dang, B.H. Hakkert, T. Vermeire, A.H. Piersma. Relative parameter sensitivity in prenatal toxicity studies with substances classified as developmental toxicants, ETS special issue 2012, accepted.

  1. Sanne A.B. Hermsen, Tessa E. Pronk, Evert-Jan van den Brandhof, Leo T.M. van der Ven, Aldert H. Piersma. Triazole-induced gene expression changes in the zebrafish embryo, ETS special issue 2012, accepted.

  2. Schulpen Sjors H.W, Joshua F. Robinson, Jeroen L.A. Pennings, Dorien A.M. van Dartel, Aldert H. Piersma. Dose response analysis of monophthalates in the murine embryonic stem cell test assessed by cardiomyocyte differentiation and gene expression, accepted.

  3. Joshua Robinson, Aart Verhoef, Vincent A van Beelen, Jeroen L Pennings, Aldert H Piersma.Dose-Response Analysis of Phthalate Effects on Gene Expression In Rat Whole Embryo Culture, TAAP, accepted.

  4. Joshua F. Robinson and Aldert Piersma. Toxicogenomic Approaches in Developmental Toxicology Testing. In: Teratogenicity Testing Methods and Protocols; Ed. Paul Barrow, 2011 Humana Press, accepted.

  5. Theunissen, Peter T, de Jong, Esther, Robinson, Joshua F, and Piersma, Aldert H. Embryonic stem cell test (EST): molecular endpoints for the high throughput analysis of the embryotoxic potential of chemicals. In: High throughput methods in toxicity screening. Wiley publishers, Pablo Steinberg, Editor., accepted.

  6. Sjors H.W. Schulpen Aldert H. Piersma The Embryonic Stem Cell Test In: Teratogenicity Testing Methods and Protocols; Ed. Paul Barrow, 2011 Humana Press, accepted.

  7. Mirjam Luijten, Amar V. Singh, Caleb Bastian, Anja Westerman, Aart Verhoef, Maia L. Green, Aldert H. Piersma, Annemieke de Vries, Thomas B. Knudsen. Altered Developmental Programming of the Mouse Mammary Gland in Female Offspring Following Perinatal Dietary Exposures; submitted.

  8. Sumitra Sengupta, Lijoy K Mathew, Lisa Truong, Esther de Jong, Aldert H. Piersma, Jane LaDu, and Robert L Tanguay. Induction of Cripto-1 by Glucocorticoid Receptor Activation Inhibits Zebrafish Fin Tissue Regeneration. PloSPlos One genetics, submitted.

  9. Kristina M. Hettne, André Boorsma, Dorien A. M. van Dartel, Jelle J.Goeman|, Esther de Jong. Aldert H. Piersma, Rob H. Stierum, Jos C. Kleinjans, Jan A. Kors. Text-mining mediated generation of chemical response-specific gene sets for gene expression-based compound potency ranking, toxic mechanism elucidation, and compound toxicity profile comparison, submitted.

  10. Virissa Lenters, Lützen Portengen, Lidwien Smit, Bo AG Jönsson, Aleksander Giwercman, Lars Rylander, Marcello Spanò, Henning S Pedersen, Jan K Ludwicki, Valentyna Zviezdai, Aldert H Piersma, Gunnar Toft, Jens Peter Bonde, Dick Heederik, Roel Vermeulen. Global screening of effects of environmental contaminants on reproductive function in Arctic and European males using partial least squares regression. Env.Health Persp. Submitted.

  11. Esther de Jong, Lianne van Beek, Aldert H. Piersma. Comparison of osteoblast and cardiomyocyte differentiation in the embryonic stem cell test for predicting embryotoxicity in vivo. Submitted.

  12. Aldert H. Piersma. Validation of alternative methods for toxicological hazard assessment – issues and challenges. AXLR8 Proceedings 2012, Submitted.

  13. Theunissen PT, Pennings JLA, van Dartel DAM, Robinson JF, Kleinjans JCS, Piersma AH Complementary detection of embryotoxic properties of substances in the neural and cardiac embryonic stem cell tests (ESTn and ESTc), Submitted.

 

RIVMRijksinstituut voor Volksgezondheid en Milieu Reports

1. Piersma AH, Peters PWJ. Toepassing van embryocarcinomacellen in het onderzoek naar werkingsmechanismen van teratogene stoffen. Annual Report 1987: 275 277.

2. Piersma AH, Verhoef A, Peters PWJ. Validatie van in vitro tests voor teratogene stoffen: de post implantatie embryokweek. Annual Report 1988: 287 289.

3. Piersma AH, Verhoef A, Jansen EHJM, van den Ham WA, Peters PWJ. Embryotoxiciteit van phenylketonurie gerelateerde metabolieten van phenylalanine in de postimplantatie ratte embryokweek. Annual Report 1989: 318 320.

4. Slooff W, et al. Basisdocument Chloorfenolen. Report no. 710401003, August 1990.

5. Garbis Berkvens JM, Hofstee Hooftman MWA, Verhoef A, Piersma AH, Peters PWJ, Koëter HWBM. Effecten van anaesthetica op de vroege ontwikkeling van de geslachtscellen van de rat. Report no. 198802001, February 1991.

6. Piersma AH, Verhoef A, Bos A, Garbis Berkvens JM, Hofstee Hooftman T, Peters PWJ. Effecten van blootstelling aan anaesthetische gassen op de ontwikkeling van post implantatie ratte embryos in vitro. Report no. 198802002, February 1991.

7. Piersma AH, Verhoef A, de Liefde A, Van Nesselrooij BPM, Garbis Berkvens JM. Foetotoxiciteit van methyleenblauw in de rat; een verkennend onderzoek. Report no. 199001001, March 1991.

8. Piersma AH, Verhoef A, de Liefde A, Van Nesselrooij BPM, Garbis Berkvens JM. Fetotoxicity of methylene blue in the rat. Annual Report 1990: 107 108.

9. Piersma AH, Haakmat AS, Hagenaars AM. New test systems for in vitro identification of teratogenic compounds as alternatives for animal experiments. Annual Report 1991: 182- 184.

10. Olling M, Bode W, Bruil M, Groen K, Piersma AH. Kinetiek en teratogeniteit van vitamine A. Een literatuurstudie. Report no. 642810001, September 1992.

11. Hessel EM, Verhoef A, van Loveren H, Piersma AH. Morphologic and immunologic effects in the rat after prenatal exposure to cyclophosphamide. Report no. 199001002, March 1993.

12. Piersma AH, Verhoef A. Congenital diaphragmatic hernia and lung hypoplasia: an animal model in the rat. Annual Report 1992: 184-186.

13. Vermeire TG, Kroese ED, Meijer GW, Mohn GRgroepsrisico, Notenboom J, Peijnenburg WJGM, Piersma AH, Roghair CJ. Initial assessment of the hazards and risks of new chemicals to man and the environment PART II. Report no. 679102018, May 1993.

14. Meijerink M, Piersma AH. Quinoclamin. Adviesrapport 93/613340/010 1e aanvulling 24-08- 93.

15. Piersma AH, Verhoef A, Hamers AM, van den Ham WA, Jansen EHJM. Maternal phenylketonuria: Embryotoxicity in vitro of PKUPhenylketonurie-related metabolites and of human PKU-sera. Report no. 199001003, November 1993.

16. Piersma AH. In vitro assays voor embryotoxiciteit. Nieuwsbrief Coördinatiepunt Aternatieven voor Dierproeven RIVM 3(1): 3-4, 1994.

17. Posthumus R, Arentzen PH, Piersma AH. Benomyl. Adviesrapport 93/623340/012 07-03-94.

18. Slooff W, et al. Basisdocument kwik. Report no. 710401023, June 1994.

19. Olling M, Piersma AH, Bruil MA, Verhoef A, Klaassen R. Toxicokinetiek en embryotoxische effecten van vitamine A na eenmalige orale toediening van verschillende doses retinylpalmitaat aan drachtige ratten. Report no. 642810002, October 1995.

20. Mennes W, Piersma AH. Volksgezondheidsaspecten van “oestrogene stoffen” in het milieu. Report no. 613320001, May 1996.

21. Mennes WC, Piersma AH. Public health aspects of estrogenic environmental contaminants: results of a limited literature survey. Annual Report 1995, 57-59.

22. Piersma AH, Verhoef A, Klaassen R, Van Eijkeren J, Olling M. Rat postimplantation embryo culture as a tool for internal dose modeling in embryotoxicity risk assessment. Report no. 604138004, April 1997.

23. Piersma AH, de Vries-Klavers CE, Jonker M. Validatie van een in vitro test batterij voor teratogeniteit van xenobiotica. Report no. 623860003, June 1997.

24. Mees MM, Cuijpers CEJ, Piersma AH. Is sperm quality actually declining? A literature survey. Report no. 650030.001, November 1997.

25. De Waal EJ, Piersma AH, Van der Stappen AJ, Verhoef A, De Jong WH, Van Loveren H. Extension of the OECD414 rat teratogenicity protocol by increasing the duration of treatment and introduction of parameters of immunotoxicity: an exploratory study using diazepam as a model compound. Report no. 316601003, February 1998.

  1. Piersma AH, Verhoef A, Elvers LH, Wester PW. Toxicity of compounds with endocrine activity in the OECD 421 reproductive toxicity screening test. Report no. 650030002, December 1998.

  2. Piersma AH. Voeding en Voortplanting. Report no. 650250003, June 1999.

28. Piersma AH, Spanjersberg MQI, Beekhuijzen MEW. Lifestyle and cancer of the reproductive organs. Report no. 650030 003, September 1999.

29. Van Loveren H, Piersma AH, De Jong WH, De Waal EJ. Developmental immunotoxicity of diazepam in prenatally treated weanling wistar rats. Report no. 640080 001, October 1999.

30. Piersma AH, Verhoef A, Dormans JAMA, Elvers LH, Valk, V de, Biesebeek, JD te, Pieters MN, Slob W. Developmental and testicular toxicity of butyl benzyl phthalate in the rat and the impact of study design. Report no. 650040001, October 1999.

31. Siemelink M, Jansen EHJM, Piersma AH, Opperhuizen A. Active components in food supplements. Report no. 650250001, April 2000.

32. Piersma AH. Endocrine disruption and human health: Workshop report on the state of the art. Report no. 650030 004, December 2000.

33. Siemelink M, Opperhuizen A, Piersma AH. Prenatal nutritional programming of adult disease. Report No. 650250 005, December 2000.

34. Piersma AH, Verhoef A, Sweep CGJ, de Jong WH, van Loveren H. Immunotoxicity and developmental toxicity in the rat after prenatal exposure to diethylstilbestrol. Report No. 650040 002, July 2001.

  1. Siemelink M, Dormans JAMA, van Loveren H, Piersma AH. Diet during pregnancy and weaning and adult disease in the offspring in the rat. Report No. 650250006/2001.

  2. Wolterink G, Piersma AH, van Engelen JGM. Risk assessment of chemicals: what about children ? Report No. 613340005/2002.

  3. Luit RJ, Beems RBRobertson-Berger, van Benthem J, Bodar CWM, van Engelen JGM, Hulzebos EM, van Loveren H, Maslankiewicz L, Piersma AH, Pronk MEJ, Rennen MAJ. Inventory of revisions in the ECEuropean Commission Technical Guidance Documents (TGD) on risk assessment of chemicals. Report No. 601200001/2003.

  4. Siemelink M, Verhoef A, vd Kuil A, Dormans JAMA, Pesman G, Piersma AH. Diabetes, stress response and blood pressure: prenatal nutritional programming in a rat model. Report No. 650100001/2003.

  5. Raaij MTM van, Janssen PAHPolycyclic aromatic hydrocarbons, Piersma AH. The relevance of developmental toxicity endpoints for acute limit setting. Report No. 601900004/2003.

  6. Schothorst F van, Piersma AH. Teratogenicity and the Threshold of Toxicological Concern Concept. Report No. 650210003/2003.

  7. Maslankiewicz L, Hulzebos EM, Vermeire TG, Muller JJA, Piersma AH. Can chemical structure predict reproductive toxicity? Report No. 601200005/2005.

  8. Pieters MN, Janssen P, Piersma A, Klaus M. Overzicht van voor de voortplanting giftige stoffen en werkplekken – een eerste aanzet. Report No. 329999001/2006.

  9. M. Luijten, A. de Vries, A. Opperhuizen, A.H. Piersma. Alternative methods in reproductive toxicity testing: state of the art. RIVM report 340720 002/2007.

  1. Vermeire T, Piersma AH, Hakkert B, et al. Selected intergated testing strategies (ITS) for the risk assessment of chemicals. Report No. 601050001/2007.

  2. Piersma AH, Hakkert B, Muller A. Current innovations in regulatory reproductive toxicity assessment of chemicals. RIVM Report 340700002/2007.

  3. W. Slob, G. Janer, J. G. Bessems, B. C. Hakkert, A. J. Sips, A. Verhoef, G. Wolterink, A. H. Piersma Quantitative in vitro – in vivo extrapolation. An analysis of 19 embryotoxic compounds. RIVM report 340720 001/2008.

  4. S. Dekkers, J. van Benthem, A.H. Piersma, P.E.D. Eysink, A.J. Baars. Ziektelast van effecten op de voortplanting ten gevolge van blootstelling aan stoffen op de werkplek. Best professional judgement. RIVM Report 320017001/2008

  5. Peter M.J. Bos, Polly E. Boon, Hilko van der Voet, Gemma Janer, Aldert H. Piersma, Beat Brüschweiler, Elsa Nielsen, Wout Slob. A semi-quantitative model for risk appreciation and risk weighing. RIVM Report ###/2008.

  6. Aldert H. Piersma. Development and implementation of alternative methods in reproductive toxicology. RIVM Report 340700005/2011.

  7. Aldert H. Piersma, Elisa C.M. Tonk, Henk van Loveren. Development of an experimental model for assessing adverse effects of juvenile chemical exposures. RIVM Report 340701001/2011.

Abstracts / Presentations

  1. Piersma AH, Ploemacher RE, Brockbank KGM. Transplantation of stromal progenitor cells (CFU F) in mice. 11th Annual Congress of the International Society for Experimental Hematology, Baltimore MD, USAUnited States of America, 1982. Exp.Hemat. 10 (suppl.11), 135, 1982.

  2. Brockbank KGM, Piersma AH, van Peer CMJ. Stimulation and inhibition of granulocyte/macrophage progenitor colony formation by activities produced by hemopoietic organ fibroblasts in vitro. 11th Symposium of the International Association for Comparative Research on Leukemia and Related Research, Cambridge UKUnited Kingdom, 1983. Leukemia Review International 1: 158 159, 1983.

  3. Brockbank KGM, Ploemacher RE, Piersma AH, Brons NHCNationaal hepatitis centrum. Longterm effects of ionizing radiation upon bone marrow stroma. 7th International Congress of Radiation Research, Amsterdam, the Netherlands, 1983. Abstract no. C1 05 in the proc. of the 7th ICRR.

  4. Piersma AH, Ploemacher RE, Brockbank KGM. Comparison of assays for measurement of radiation damage to hemopoietic stroma. 7th International Congress of Radiation Research, Amsterdam, the Netherlands, 1983. Abstract no. C1 25 in the proc. of the 7th ICRR.

  5. Brockbank KGM, Piersma AH, van Peer CMJ. An in vitro analysis of the number and spatial distribution of murine femoral marrow fibroblastoid progenitors and fibroblastoid cell function during aging. 12th Annual Congress of the International Society for Experimental Hematology, London UK, 1983. Exp.Hemat. 11 (suppl.14), 264, 1983.

  6. Piersma AH, Brockbank KGM, Ploemacher RE. Regulation of in vitro myelopoiesis by a hemopoietic stromal fibroblast cell line. 12th Annual Congress of the International Society for Experimental Hematology, London UK, 1983. Exp.Hemat. 11 (suppl.14), 266, 1983.

  7. Piersma AH, Brockbank KGM, Ploemacher RE. Transplantation kinetics of fibroblastic colony forming cells in murine bone marrow. 13th Annual Congress of the International Society for Experimental Hematology, Atlanta GA, USA, 1984.

  8. Brockbank KGM, Piersma AH, Voerman J. Colony stimulating activity production by bone marrow derived adherent cells. 13th International Congress of the International Society for Experimental Hematology, Atlanta GA, USA,1984.

  9. Piersma AH, Brockbank KGM, Ploemacher RE. Transplantation of hemopoietic stromal progenitor cells by blood transfusion in mice. 18th Congress of the International Society for Blood Transfusion. Munich FRG, 1984.

  10. Piersma AH, Ploemacher RE, Brockbank KGM, Ottenheim CPE. A specific determinant on reticular cells in murine embryonic and adult hemopoietic stroma. 14th Annual Congress of the International Society for Experimental Hematology, Jerusalem, Israel, 1985.

  11. Ploemacher RE, Piersma AH, Brockbank KGM, Nikkels PGJ, Ottenheim CPE. Migration of fibroblastoid stromal cells in murine blood. 14th Annual Congress of the International Society for Experimental Hematology, Jerusalem, Israel, 1985.

  12. Brockbank KGM, de Jong JP, Piersma AH, Voerman J. Maintenance of hemopoietic stem cells and production of differentiated progeny on murine bone marrow derived reticular fibroblast monolayers in vitro. 14th International Congress of the International Society for Experimental Hematology, Jerusalem, Israel, 1985.

  13. De Jong JP, Willemsen R, Piersma AH, Nikkels PGJ, Voerman JSA, Ploemacher RE. Distribution of a monoclonal antibody raised against reticular cells from murine bone marrow. 15th Annual Congress of the International Society for Experimental Hematology, Buffalo, USA, 1986.

  14. Piersma AH, Willemse A, Mummery CL, de Laat SW. Identification of teratogenic substances using differentiating embryocarcinoma cells in vitro. 26th Annual Meeting of the Teratology Society, Boston MA, USA, 1986; and 14th Conference of the European Teratology Society, Helsingør, Denmark, 1986.

  15. Piersma AH, Willemse A, De Laat SW, Mummery CL. An in vitro model system for mesoderm formation in embryonic development: Differentiation of embryonal carcinoma cells in coulture with visceral endoderm cell lines. 15th Conference of the European teratology Society, Fribourgh, Switzerland, 1987. Prize winning presentation

  16. Piersma AH, Willemse A, de Laat SW, Mummery CL. Mesodermal differentiation of embryonal carcinoma cells in coculture with their endodermal derivatives. European Developmental Biology Congress, Helsinki, Finland, 1987; and NATO Advanced Research Workshop: Cell to Cell Signals in Mammalian Development, Les Arcs, France, 1988.

  17. Piersma AH, van Achterberg T, de Laat SW, Mummery CL. Embryonal carcinoma cell differentiation in in vitro models for endoderm and mesoderm formation: modulation by teratogenic compounds. 28th Annual Meeting of the Teratology Society, Palm Beach FL, USA, 1988.

  18. Piersma AH, Achterberg T van, Roest P, Mummery CL, Peters PWJ. Cadmium-induced inhibition of proliferation and differentiation of embryonal carcinoma cells in culture is counteracted by zinc. 17th Annual Meeting of the European Teratology Society, Budapest, Hungary, 1989.

  19. Van Aerts LAGJM, Piersma AH, Verhoef A, Peters PWJ, Copius Peereboom Stegeman JHJ. Bioactivation of cyclophosphamide in an in vitro post implantation rat embryo/hepatocytes coculture, using maternal hepatocytes in suspension. Symposium Dutch Society of Toxicology, Utrecht, 17 march 1989; and 17th Annual Meeting of the European Teratology Society, Budapest, Hungary, 1989; and International Conference on in vitro Culture of Postimplantation Rodent Embryos, Louvain la Neuve, Belgium, 1990.

  20. Govers MJAP, Attenon P, Van Maele Fabry G, Peters PWJ, Picard J, Piersma AH, Schmid BP, Stadler J, Verhoef A, Verseil C. Interlaboratory validation of rodent post implantation embryo culture as a screening test for teratogenic compounds. 29th annual meeting of the Teratology Society, Richmond VA, USA, 1989; and 17th Annual Meeting of the European Teratology Society, Budapest, Hungary, 1989.

  21. Schmid BP, Attenon P, Govers MJAP, Van Maele Fabry G, Peters PWJ, Picard JJ, Piersma AH, Stadler J, Verhoef A, Verseil C. Rodent post  implantation embryo culture as a screening test for teratogenic compounds: interlaboratory validation studies. 2nd International Conference on Practical in vitro Toxicology, Nottingham, UK, 1989.

  22. Peters PWJ, Piersma AH. In vitro embryotoxicity and teratogenicity studies. 2nd International Conference on Practical in vitro Toxicology, Nottingham, UK, 1989.

  23. Piersma AH, van Achterberg T, Mummery CL, de Laat SW. Mesodermal differentiation of embryonal carcinoma cells in coculture with visceral endoderm cell lines. 11th International Congress of the International Society of Developmental Biologists, Utrecht, The Netherlands, 1989.

  24. Mummery CL, van den Eijnden J, Freund E, Slager H, Piersma AH, Feijen A, Kruijer W. Regulation of growth and differentiation in early development. 11th International Congress of the International Society of Developmental Biologists, Utrecht, The Netherlands, 1989.

  25. Piersma AH, van Achterberg T, Roest P, Mummery CL, Peters PWJ. Cadmium induced inhibition of proliferation and differentiation of embryonal carcinoma cells in culture is counteracted by zinc. 17th Annual Meeting of the European Teratology Society, Budapest, Hungary, 1989.

  26. Hamers AEP, Piersma AH, Verhoef A, Jansen EHJM, Peters PWJ. Embryotoxicity of phenylketonuria(PKU) related metabolites of phenylalanine (phePublic Health England) in rat whole embryo culture. 17th Annual Meeting of the European Teratology Society, Budapest, Hungary, 1989; and International Conference on in vitro Culture of Postimplantation Rodent Embryos, Louvain la Neuve, Belgium, 1990. Reproductive Toxicology 5:266,1991.

  27. Van den Ham WA, Jansen EHJM, Piersma AH. Determination of phenylalanine metabolites in relation to maternal phenylketonuria. Pharmaceutisch Weekblad Scientific edition, 11 1989, N9.

  28. Piersma AH, Attenon J, Bechter R, Govers MJAP, van Maele Fabry G, Peters PWJ, Picard J, Schmid BP, Stadler J, Verhoef A, Verseil C. Rodent post implantation embryo culture: Interlaboratory validation as a screening test for teratogenic compounds. International Conference on in vitro Culture of Postimplantation Rodent Embryos, Louvain la  Neuve, Belgium, 1990. Reproductive Toxicology 5:267,1991.

  29. Piersma AH, Verhoef A, Jansen EHJM, van den Ham WA, Peters PWJ. Embryotoxicity of phenylketonuria related metabolites of phenylalanine in rat whole embryo culture. 31st Dutch Federation Meeting, Leiden, The Netherlands, 1990.

  30. Hessel EM, Verhoef A, Piersma AH, Peters PWJ. Morphologic and immunologic effects in the rat of prenatal exposure to cyclophosphamide. 30th Annual Meeting of the Teratology Society, Victoria, Canada, 1990.

  31. Piersma AH, van Aerts LAGJM, Verhoef A, Robinson JE, Peters PWJ. Biotransformation in post implantation rat embryo culture using maternal hepatocytes in suspension coculture. 31st Annual Meeting of the Teratology Society, Victoria, Canada, 1990.

  32. Piersma AH, Verhoef A, Jansen EHJM, van den Ham WA, Peters PWJ. Phenylketonuria related metabolites of phenylalanine are embryotoxic in vitro. 30th Annual Meeting of the Teratology Society, Victoria, Canada, 1990.

  33. Piersma AH, Verhoef A, De Liefde A, Van Nesselrooij BPM, Garbis Berkvens JM. Embryotoxicity of methylene blue in the rat. 32nd Dutch Federation Meeting, Amsterdam, The Netherlands, 1991; 31st Annual Meeting of the Teratology Society, Boca Raton FL, USA, 1991; Symposium Risk Assessment in Teratology and Genetic Toxicology, Pilsen, Czechoslowakia, 1991; 19th Annual Meeting of the European Teratology Society, Antwerp, Belgium, 1991.

  34. Piersma AH, Haakmat AS, Hagenaars AM. In vitro assays for the identification of compounds interfering with embryonic cell differentiation using embryonal carcinoma cells. 20th Annual Meeting of the European Teratology Society, Würzburg, Germany, 1992.

  35. Piersma AH. Whole embryo culture and toxicity testing. 3d Symposium on Whole Embryo Culture, Basel, Switzerland, 1992.

  36. Piersma AH, Hofstee-Hooftman MWA, Verhoef A. Early lung development in the rat and its perturbation by nitrofen treatment. 33d Annual Meeting of the Teratology Society, Tucson AZ, USA, 1993; 21st Annual Meeting of the European Teratology Society, Lyon, France, 1993.

  37. Piersma AH, Garbis-Berkvens JM, Rost van Tonningen-van Driel MM, Peters PWJ. Informatieverstrekking over teratogene en reproduktietoxische stoffen. Annual Meeting of the Dutch Pediatric Society, Veldhoven, The Netherlands, 1993.

  38. Piersma AH. Maternal Toxicity and risk assessment in developmental toxicology. Symposium on Health Hazards of Glycol Ethers, INRS, Nancy, France, 1994.

  39. Brandsma AEalveolaire echinokokkose, Piersma AH, Hofstee-Hooftman TWA, Verhoef A, TenHave-Opbroek AAWAlgemene arbeidsongeschiktheidswet, Tibboel D. Early lung development in the rat and its perturbation by nitrofen exposure. Pediatric Week Holland, Rotterdam, The Netherlands, 1994.

  40. Piersma AH, Verhoef A, Olling M. Transient delay in neural tube closure in rat embryos after a single oral dose of retinylpalmitate. 22nd Annual Meeting of the European Teratology Society, Prague, Czech Republic, 1994.

  41. Verhoef A, Bechter R, Krafft N, Piersma AH, Schmid BP, Stadler J, Verseil C, Zijlstra JA. Interlaboratory evaluation of five pairs of xenobiotic compounds in the postimplantation embryo culture. 4th Symposium on Vertebrate Whole Embryo Culture, Milan, Italy, 1994.

  42. Piersma AH, Verhoef A, Dortant PM. Evaluation of the OECD 421 reproductive toxicity screening test using Butyl Benzyl Phthalate (BBPBruto Binnenlands Product). Annual Meeting of the Dutch Society of Toxicology, Utrecht, The Netherlands, 1995, Human and Experimental Toxicology 14: 689, 1995; 23d Annual Conference of the European Teratology Society, Dublin, Ireland, 1995.

  43. Piersma AH, Verhoef A, Olling M. A single oral dose of retinylpalmitate may cause a transient delay in neural tube closure in rat embryos. 35th Annual Meeting of the Teratology Society, Newport Beach CA, USA, 1995, and 36th Annual Meeting of the Teratology Society, Keystone CO, USA, 1996.

  44. Piersma AH, Jonker M. Validation of differentiating embryocarcinoma cell cultures as assays for developmental toxicants. 2nd World Congress on Alternatives and Animal Use in the Life Sciences, Utrecht, The Netherlands, 1996. ATLA 24: 275, 1996.

  45. Verhoef A, Olling M, Van Eijkeren J, Piersma AH. Rat postimplantation whole embryo culture as a tool in internal dose modeling in developmental toxicology. 2nd World Congress on Alternatives and Animal Use in the Life Sciences, Utrecht, The Netherlands, 1996. ATLA 24: 295, 1996; and Vth International Symposium on Vertebrate Whole Embryo Culture, Jerusalem, Israel, April 1-4, 1997.

  46. Piersma AH, Verhoef A, Olling M, Van Eijkeren J. Carbamazepine toxicity in rat postimplantation embryo culture after in vitro exposure via three routes. 25th Conference of the European Teratology Society, Cannes, France, 1997.

  47. Piersma AH, Verhoef A, Elvers LH, Wester P. Toxicity of compounds with endocrine activity in the OECD421 reproductive toxicity screening test. 38th Annual Meeting of the Teratology Society, San Diego CA, USA, 1998, Teratology 57: 243-244, 1998.

  48. Scholz G, Pohl I, Seiler A, Bremer S, Brown N, Piersma A, Holzhutter HG, Genschow E, Spielmann H. Results of the first phase of the ECVAM Project “Prevalidation and validation of three in vitro embryotoxicity tests”. 26thConference of the European Teratology Society, Stockholm, Sweden, 1998.

  49. Beekhuijzen MEW, Verhoef A, Klaassen R, Rompelberg CJM, Piersma AH. Pharmacokinetics and developmental toxicity in vivo and in vitro of phenytoin in the rat. 27th Conference of the European Teratology Society, Oxford UK, 1999.

  50. Piersma AH, Verhoef A, te Biesebeek JD, Pieters MN, Slob W. Developmental toxicity study of butylbenzylphthalate in the rat using a multiple dose study design. 40th Annual Meeting of the Teratology Society, Palm Beach FL, USA, 2000, and 28th Conference of the European Teratology Society, Ferrara, Italy, 2000.

  51. Peters PWJ, Blok EN, Piersma AH. Is voedselverrijking nodig? Symposium Voeding en Zwangerschap, St. Obstetrie en Gynaecologie Research, Nijmegen, 1 december 2000.

  52. Siemelink M, Verhoef A, Opperhuizen A, Piersma AH. Perinatal nutrition and postnatal development in the rat. 40th Annual Meeting of the Society of Toxicology, San Francisco CA, USA, 2001

  53. Moerbeek M, Piersma AH, Slob W. Is the current study design suitable for assessing the benchmark dose? Society for Risk Analysis Annual Meeting, Seattle WA, USA, 2001.

  54. Spielmann H, Genschow E, Scholz G, Seiler A, Brown NA, Piersma AH, Brady M, Elemann N, Huuskonen H, Paillard F, Bremer S, Becker K. Results of the ECVAM validation study of three in vitro embryotoxicity tests. Reproductive Toxicology 15: 461, 2001; 29th Conference of the European Teratology Society, Balatonfured, Hungary, 2001

  55. Piersma AH. Validation of in vitro tests for embryotoxicity. 29th Conference of the European Teratology Society, Balatonfured, Hungary, 2001.

  56. Piersma AH. EU Guideline on Classification and Labelling of Substances toxic to reproduction (Directive 67/548/EEC). 29th Conference of the European Teratology Society, Balatonfured, Hungary, 2001.

  57. Piersma AH. Endocrine Disruption. Chemische Kring Zwolle and Open University, Zwolle, The Netherlands, 12 March 2002.

  58. Siemelink M, Verhoef A, Piersma AH. Maternal nutrition and postnatal development of the offspring in the rat. 42nd Annual Meeting of the Teratology Society, Scottsdale, Arizona, USA, 2002, and 30th Conference of the European Teratology Society, Hannover, Germany, 2002.

  59. Piersma AH, Siemelink M, Verhoef A, Dormans JAMA, Span PN. Dietary fatty acid composition during pregnancy in the rat programs growth and glucose metabolism in the offspring. 34th Annual Meeting of the Diabetic Pregnancy Study Group, Balaton, Hungary, 2002, and 2nd World Congress on Fetal Origins of Adult Disease, Brighton UK, June 2003.

  60. Piersma AH. Endocrine active compounds and human health risk. Annual Meeting of the Netherlands Society of Toxicology, Utrecht, 16 January 2003.

  61. Piersma AH. Is separate consideration of teratogenicity a necessity in the application of the TTC? ILSIInternational Life Sciences Institute-Workshop on Structure-based thresholds of toxicological concern: guidance for application to substances present at low levels in the diet, Vienna, Austria, 20-21 March 2003.

  62. Luijten M, vandenBerg-Wijnands J, Nagelkerke N, Verhoef A, Piersma AH, Wester P, van Kranen HJ Rao GN, van Kreijl CF. Accelleration of spontaneous mammary tumor development in Tg.NK mice by a high risk western diet. 2nd World Congress on Fetal Origins of Adult Disease, Brighton UK, June 2003.

  63. Piersma AH, van Loveren H. Developmental Immunotoxicology and test guidelines. ETS Conference, 7-10 September 2003, Elsinore, Denmark.

  64. Piersma AH, Westerhof M, Westerman A, Verhoef A. Caffeine enhances the embryotoxicity of antiepileptic drugs in rat postimplantation whole embryo culture. ETS Conference, 7-10 September 2003, Elsinore, Denmark.

  65. Piersma AH. In vitro tests for embryotoxicity. EUROTOX Conference, 28 September – 1 October 2003, Florence, Italy.

  66. Van Loveren H and Piersma AH. Immunotoxicological consequences of perinatal chemical exposures. EUROTOX Conference, 28 September – 1 October 2003, Florence, Italy.

  67. Mirjam Luijten, Anni Rønfeldt Thomsen, Jolanda van den Berg, Piet Wester, Aart Verhoef, Nico J.D. Nagelkerke, Adile Samaletdin, Ritva Takkinen, Herman Adlercreutz, Aldert Piersma, Ilona K. Sørensen, Ghanta N. Rao(†), Henk J. van Kranen and Coen F. van Kreijl. Soy-derived isoflavones and a high-fat Western diet enhance spontaneous mammary tumor development in Tg.NK mice. Karlsruher Ernahrungstage, 11-14 October 2003, Karlsruhe, Germany, and 24th Congress of the International Association for Breast Cancer Research, 1-5 November 2003, Sacramento CA, and European Teratology Society Conference, Thessaloniki, Greece, 19-22 September 2004.

  68. Piersma AH. Reproductive Toxicology and REACH. PAON Postacademic CourseClimate and Ozone change effects on Ultraviolet radiation and risks using Remote sensing based System Evaluations, 12 November 2003, Utrecht, The Netherlands.

  69. S. Germer, A. H. Piersma , L. van der Ven, H.-J.Schmitz and D.Schrenk EFFECT OF TETRABROMO-BISPHENOL A (TBBPA), A FLAME RETARDANT, ON EXPRESSION OF CYTOCHROME P450 ISOZYMES IN RAT LIVER Annual Meeting of the German Society of Experimental and Clinical Pharmacology and Toxicology in Mainz, March 2004.

  70. Meeuwen, J van, Piersma AH, Berg, M van den, Sanderson T. Lack of synergistic or antagonistic effects of a mixture of phytoestrogens on cell proliferation of MCF-7 human breast cancer cells (E-screen). Poster abstract number 582, SOT Baltimore, March 2004.

  71. Piersma AH. Reproductive toxicity models, data and interpretation. Refresher Course for Clinical Pharmacologists in Medical Ethical Review Committees, The Hague, The Netherlands, 13 April, 2004.

  72. Meeuwen J van, Piersma AH, van den Berg M, Sanderson JT. Effects of mixtures of estrogenic chemicals on uterine growth of prepubertal rats (uterotrophic assay). Jubilee Annual Meeting of the Netherlands Toxicology Society, Veldhoven NL, June 2004.

  73. Meeuwen J van, Piersma AH, van den Berg M, Sanderson JT. Additive effects of mixtures of phytochemicals on cell proliferation of MCF-7 human breast cancer cells (E-screen). Jubilee Annual Meeting of the Netherlands Toxicology Society, Veldhoven NL, June 2004.

  74. Raaij MTM van, Janssen PAH, Piersma AH. The selection and relevance of reproductive toxicity endpoints for acute exposure limit setting. Jubilee Annual Meeting of the Netherlands Toxicology Society, Veldhoven NL, June 2004.

  75. Raaij, M van, Janssen, PAH, Piersma AH. The selection and relevance of developmental toxicity end points for acute exposure limit setting. Jubilee Annual meeting of the Netherlands Toxicology Society, Veldhoven NL, June 2004.

  76. Germer S, Piersma AH, van der Ven L, Schmitz H-J, Schrenk D. Influence of the Flame Retardant Tetrabromobisphenol-A on the Expression of Cytochrome P450 Isoenzymes in Rat Liver. Dioxin 2004, Berlin

  77. Piersma AH, Verhoef A, Dormans JAMA. Dietary fatty acids during pregnancy in the rat and glucose homeostasis in the offspring. NUGO week Wageningen, 6-10 september 2004.

  78. Luijten M, Verhoef A, Dormans JAMA, Beems D, Van Kranen HJ, Nagelkerke NJD, Adlercreutz H, Piersma AH. Phytoestrogens and dietary fatty acids influence spontaneous mammary tumor development in in Tg.NK (MMTV/c-neu) mice. SOT New Orleans March 2005.

  79. van Meeuwen, J.A., van den Berg, M., Sanderson, J.T., Piersma A.H. Mixtures of exogenous estrogens cause additive uterotrophic effcets in pre-pubertal rats. Proceedings of the scientific meeting of the Netherlands Society of Toxicology, Oss, The Netherlands, 2005.

  80. van Meeuwen, J.A., Korthagen, N., de Jong, P.C., Piersma A.H., van den Berg, M. Effects of phytochemicals on co-cultures of MCF7 and primary human mammary fibroblasts. Proceedings of the scientific meeting of the Netherlands Society of Toxicology, Oss, The Netherlands, 2005.

  81. Luijten, M, Westerman A, Verhoef A, Piersma AH. Gene Expression Changes In Rat Embryos Exposed In Vitro To Retinoic Acid. Teratology Society Meeting, St.sintPete’s Beach, FL, USA, 25-30 June 2005.

  82. Silke Germer, Aldert H. Piersma, Leo T.M. van der Ven, Andreas Kamyschnikow, Hans-Joachim Schmitz and Dieter Schrenk. Effects of hexabromocyclododecane (HBCD) on gene expression of cytochromes P450 in rat liver. Dioxin 2005.

  83. Mirjam Luijten*, Anja Westerman, Aart Verhoef, Aldert H. Piersma Gene expression changes in rat embryos exposed in vitro to retinoic acid. European Teratology Society Conference, Haarlem, The Netherlands, 3-7 September 2005.

  84. Mirjam Luijten, Aart Verhoef, Jan A.M.A. Dormans, Rudolf B. Beems, Henk J. van Kranen, Nico J.D. Nagelkerke, Herman Adlercreutz, Aldert H. Piersma. Phytoestrogens and dietary fatty acids influence spontaneous mammary tumor development in TG.NK (mmtv/c-neu) mice. European Teratology Society Conference, Haarlem, The Netherlands, 3-7 September 2005.

  85. Lidka Maslankiewicz, Etje Hulzebos, Theo Vermeire, Andre Muller and Aldert Piersma. Can chemical structure predict reproductive toxicity? European Teratology Society Conference, Haarlem, The Netherlands, 3-7 September 2005.

  86. Jeroen van Meeuwen, Martin van den Berg, Thomas Sanderson, Aldert H. Piersma. Mixtures of exogenous estrogens cause additive uterotrophic effects in prepubertal rats. European Teratology Society Conference, Haarlem, The Netherlands, 3-7 September 2005.

  87. Leo van der Ven, Hellmuth Lilienthal, Aldert Piersma, Aart Verhoef, Joseph Vos Endocrine disrupting and neurobehavioural effects of the brominated flame retardant TBBPA in a reproduction toxicity study in rats. European Teratology Society Conference, Haarlem, The Netherlands, 3-7 September 2005, Gordon Conference on Endocrine Disruption, Il Ciocco, Lucca, Italy, 5-8 June 2006.

  88. J. van Meeuwen, A. Piersma, M. VandenBerg Effects of phytoestrogens on cocultures of the MCF7 mammary tumor cell line and primary human fibroblasts. 45thAnnual meeting of the Society of Toxicology, San Diego CA, March 5-9, 2006.

  89. Lilienthal H, van der Ven L, Hack A, Roth-Harer A, Piersma A, Vos J. Neurobehavioural effects in relation to endocrine alterations caused by exposure to borminated flame retardants in rats. SETAC 2006.

  90. Lilienthal H, van der Ven L, Roth-Härer A, Hack A, Piersma A, Vos J Neurobehavioural toxicity of bominated flame retardants: differential effects of PBDEpolybroomdifenylethers-99, TBBPA and HBCD and endocrine relation Dioxin 2006.

  91. Silke Germer, Yvonne Fery, Andreas Kamyschnikow, Aldert H. Piersma, Leo T.M. van der Ven, Dieter Schrenk. Pentabrominated Diphenylether induces Cytochrome P450 enzymes in MAle and Female Rats. Dioxin 2006.

  92. Van der Ven LTM, van de Kuil A, verhoef A, fernandez-Canton R, Germer S, Lilienthal H, Schrenk D,van den Berg M, Piersma AH, Leonards, P, Vos, JG Endocrine disrupting effects of selected brominated flame retardants in rats. Dioxin 2006.

  93. Lilienthal H, van der Ven L, Hack A, Roth-Härer A, Piersma A, Vos J. Altered Neurobehavioral Functions in Rats after Developmental Exposure to Endocrine-active Environmental Contaminants - Brominated Flame Retardants. FENS 2006.

  94. Mirjam Luijten*, Aart Verhoef, Anja Westerman, Aldert H. Piersma. Application of a metabolising system as an adjunct to the whole embryo culture protocol. European Teratology Society 34thConference 3-6 September 2006, Padova, Italy. Reproductive Toxicology 22 (2006), 276 (abs).

  95. Mirjam Luijten1*, Aart Verhoef1, Ferdi van der Horst1, Jeroen L.A. Pennings1, Jan A.M.A. Dormans1, Rudolf B. Beems1, Henk J. van Kranen1, Nico J.D. Nagelkerke2, José L. Peñalvo3, Herman Adlercreutz3, Aldert H. Piersma1. Dietary fatty acids and phytoestrogens modulate spontaneous mammary tumor development in TG.NK (MMTV/C-neu) mice. European Teratology Society 34thConference 3-6 September 2006, Padova, Italy Prize winning presentation. Reproductive Toxicology 22 (2006), 275 (abs).

  96. Marc F.J. Renkens*, Mirjam Luijten, Anja Westerman, Aart Verhoef, Frederik-Jan van Schoten*, Aldert H. Piersma. Assessment of the sensitivity of differential gene expression profiles as endpoint for developmental toxicity in rat Postimplantation whole embryo culture. European Teratology Society 34thConference 3-6 September 2006, Padova, Italy Prize winning presentation. Reproductive Toxicology 22 (2006), 282 (abs).

Bessems J., Slob W, Van Eijkeren J, Sips, Hakkertand A. Piersma Postimplantation Rat Whole-Embryo Culture assay: In vitro – In VivoExtrapolation. InVitTox 2006, Ostende, Belgium.

Bessems, Jos; Slob, Wout; Piersma, Aldert; Hakkert, Betty; Sips, AdriennePostimplantation rat whole embryo culture assay: in vitro – in vivo extrapolation. SOT Charlotte NC USA March 2007.

  1. Piersma AH. Alternatieven in de reproductietoxicologie. Voordracht Publieksdag Dierenbescherming afdeling Utrecht i.s.m Nederlands Centrum voor Alternatieven en Faculteit Diergeneeskunde Universiteit Utrecht. 24 April 2007. Lef In Het Lab Publieksprijs. Prize winning presentation

  2. Leo TM van der Ven, Hellmuth Lilienthal, Ton van de Kuil, Aldert H Piersma. Endocrine effects of hexabromocyclododecane (HBCD) in a one-generation reproduction study in Wistar rats. Teratology Society 2007.

  3. Janer, Piersma ea A retrospective analysis of the two-generation study: What is the added value of the second generation? Teratology Society 2007, European Teratology Society Bratislava, Slowakia, September 2007.

  4. Singh, Piersma ea Altered Developmental (Fetal) Programming of the Mouse Mammary Gland in Female Offspring Following Maternal Dietary Exposures. Teratology Society Pittsburgh PA, June 2007.

  5. Leo van der Ven, Raoul Kuiper, Piet Wester, Hellmuth Lilienthal, Aldert Piersma Evaluation of effects on development and reproduction via endocrine disrupting using in vivo rat and zebrafish toxicology models: the case of the brominated flame retardant TBBPA. International Conference on fetal programming and developmental toxicity, Torshavn, Faroe, 20-24 May 2007

  6. Leo van der Ven, Dorien Verhallen, Aart Verhoef, Wout Slob, Aldert Piersma. Alternative in vitro test methods for developmental toxicity testing. International Conference on fetal programming and developmental toxicity, Torshavn, Faroe, 20-24 May 2007.

  7. Gemma Janer, Wout Slob, Betty Hakkert, Theo Vermeire, Aldert Piersma. A retrospective analysis of developmental toxicity studies: what is the added value of the rabbit as an additional test species? European Teratology Society, Bratislava, Slowakia, September 2007.

  8. Hellmuth Lilienthal, Leo van der Ven, Aldert Piersma and Joseph Vos. Analysis of benchmark doses to assess effects of brominated flame retardants on neurophysiological and behavioral responses in rats. Second Workshop on Low Dose Effects of Chemicals with Special Consideration of Phthalates, Berlin, April 26-28, 2007.

  9. Hellmuth Lilienthal, Leo van der Ven, Aldert Piersma and Joseph Vos Auditory and neurobehavioural effects of exposure to brominated flame retardants in rats – evaluation of benchmark doses. European Teratology Society, Bratislava, Slowakia, September 2007.

  10. C. Bastian, T. Knudsen, A. Singh, M. Luijten, A. De Vries, and A. Piersma. Systems-Based Analysis of Developmental Programming of Mammary Gland using Bayesian-Variable-Selection. 37th Annual meeting of the Int. Soc. Dental Research, Dallas, USA, April 2-5, 2008. Prize winning presentation

  11. Vincent van Beelen, Mirjam Luijten, Anja Westerman, Aart Verhoef, Jeroen Pennings, Frederik-Jan van Schooten, Aldert Piersma. Embryotoxicity of retinoic acid in postimplantation rat whole embryo culture monitored with gene expression analysis. SOT Seattle WA, USA, March 16-20, 2008.

  12. Aldert Piersma. A retrospective analysis of the two-generation study: what is the added value of the second generation? SOT Seattle WA, USA, March 16-20, 2008.

  13. Piersma AH. Reproductive toxicology, REACH and testing strategies. British Toxicology Society autumn meeting, Liverpool, 8-9 September 2008.

  14. Margriet Park, Wytske Annema, Anna Salvati, Iseult Lynch, Kenneth Dawson, Aldert Piersma en Wim de Jong. Size dependent cytotoxicity and inhibition of differentiation of mouse embryonic stem cells by well-characterized SiO2 nanoparticles. Nanotoxicology conference abstract Zurich, 7-10 September 2008.

  15. Vincent van Beelen, Mirjam Luijten, Anja Westerman, Aart Verhoef, Jeroen Pennings, Frederik-Jan van Schooten, Aldert Piersma. Embryotoxicity of retinoic acid in postimplantation rat whole embryo culture: a genomics approach. EuropeanTeratology Society Annual Conference, Edinburgh, 21-24 September 2008.

  16. Dorien Verhallen, Peter J. Hendriksen, Jeroen L. Pennings, Frederik-Jan van Schooten, Aldert H. Piersma. Gene expression changes in differentiating embryonic stem cells induced by monobutylphthalate EuropeanTeratology Society Annual Conference, Edinburgh, 21-24 September 2008.

  17. Piersma AH. Reproductive toxicology, REACH and testing strategies. SETAC Brussels, 23-24 october 2008.

  18. Piersma AH. Reproductive risks. Masterclass Occupational Toxicology, Nijmegen University, 6 november 2008.

  19. Piersma AH. Reproductive toxicology, REACH and testing strategies. Industry Developmental toxicity Research Group, Midlands, UK, 12-13 november 2008.

  20. Piersma AH. Beoordeling van reproductietoxiciteit van chemische stoffen: de centrale plaats van de blootstellingsperiode in de reproductiecyclus. Voordracht Contactgroep Chemie, NOTOX, Dem Bosch, 12 March 2009.

  21. De Groot, Didima M.; De Groot, M.; De Esch, C.; Tonk, I.; Lammers, J.; Van Loveren, L.; Piersma, A.; Waalkens, I.; Wolterbeek, A.; Woutersen, R. Evaluation of physical, sensory and sexual landmarks in an extended one generation reproduction study: a comparison between classical NOAELno observed adverse effect level and a benchmark approach. SOT Baltimore MD, USA, 16-19 March 2009.

  22. Piersma AH. Embryonic stem cells and transcriptomics in developmental toxicity testing. INVITROM 3d Symposium, Den Bosch NL, 26 March 2009

  23. Piersma AH. The extended one-generation reproduction toxicity study: saving animals and improving hazard assessment? NVT jaarvergadering, Veldhoven NL, June 2009.

  24. Sanne A.B. Hermsen* 1 2 3, Evert-Jan van den Brandhof1, Leo T.M. van der Ven1, Aldert H. Piersma1 3Relative embryotoxicity of glycolether metabolites in a modified zebrafish embryotoxicity assay Abstract 37th Annual ETS Conference Arles, France, September 2009

  25. Janer G, Slob W, Hakkert B, Vermeire T, Piersma AH A retrospective analysis of developmental toxicity studies in rat and rabbit: value of the rabbit as an additional test species and factors that influence this assessment Abstract 37th Annual ETS Conference Arles, France, September 2009

  26. Tonk ECM, DMGDepartment of Sustainability, Environment and health de Groot, AH Penninks, DH Waalkens-Berendsen, APM Wolterbeek, AH Piersma, H van Loveren. Developmental Immunotoxicity Of Methylmercury: The Relative Sensitivity Of Developmental And Immune Parameters. Teratology Society 49th Annual Meeting, Puerto Rico, USA, June 2009, and ETS 37th Annual Conference Arles, France, September 2009. Prize winning presentation

  27. Theunissen PT, van Dartel DAM, Schulpen SHW, Hermsen SAB, van Schooten FJ, Piersma AH. Multilineage Neural Differentiation Of Murine Embryonic Stem Cells As An Alternative Test Model In Developmental Toxicology. Teratology Society 49th Annual Meeting, Puerto Rico, USA, June 2009 and 37th Annual ETS Conference Arles, France, September 2009.

  28. D.A.M. van Dartel, A. Boorsma, J.L. Pennings, F.J. van Schooten, A.H. Piersma. Transcriptomics-based Identification of Developmental Toxicants through their Interference with Cardiomyocyte Differentiation of Embryonic Stem Cells. Teratology Society 49th Annual Meeting, Puerto Rico, USA, June 2009 and 37th Annual ETS Conference Arles, France, September 2009. Prize winning presentation

  29. Esther de Jong, Jochem Louisse, Miriam Verwei, Bas J. Blaauboer, Han van de Sandt, Ruud A. Woutersen, Ivonne M.C.M. Rietjens, Aldert H. Piersmaa. Relative Developmental Toxicity Of Glycol Ethers In The Embryonic Stem Cell Test And Comparison To Their In Vivo Potency Ranking. Teratology Society 49th Annual Meeting, Puerto Rico, USA, June 2009 and 37th Annual ETS Conference Arles, France, September 2009.

  30. Jochem Louisse, Esther de Jong, Johannes J.M. van de Sandt, Bas J. Blaauboer, Aldert H. Piersma, Ivonne M.C.M. Rietjens, Miriam Verwei. The use of in vitro toxicity data and Physiologically Based Kinetic modeling to predict in vivo embryotoxic effect levels. 7th World Congress on Alternatives & Animal Use in the Life Sciences, Rome, August 2009.

  31. Piersma AH Computational Toxicology: Challenges for Regulatory Toxicology and Legislation. Teratology Society 49th Annual Meeting, Puerto Rico, USA, lecture, 2 July 2009.

  32. Piersma AH Whole embryo cultures. ILSI HESI DART ETS satellite symposium lecture, Arles, 10 Sept 2009.

  33. Theunissen PT, van Dartel DAM, Kleinjans JCS, van Schooten FJ, Piersma AH. Chemische veiligheid en het ongeboren kind. Een nieuwe, snelle en goedkope test zonder proefdieren. Poster STW marketing bijeenkomst, Den Bosch, 8 Oktober 2009.

  34. Piersma A.H. Reproductive Toxicology and Chemicals Regulation in Europe. Course Lecture, SOT, 49th Annual Meeting, Salt Lake City, 7-11 March 2010.

  35. Dorien A.M. van Dartel, Jeroen L.A. Pennings, Tessa E. Pronk, Marcel H. van Herwijnen, Joost H. van Delft, Frederik J. van Schooten, Aldert H. Piersma Transcriptomics-Based Identification of Developmental Toxicants by Their Modulation of Early Embryonic Stem Cell Differentiation. SOT, 49th Annual Meeting, Salt Lake City, 7-11 March 2010.

  36. ECM Tonk, DMG de Groot, AH Penninks, DH Waalkens-Berendsen, APM Wolterbeek, AH Piersma, H van Loveren Developmental Immunotoxicity Of Methylmercury: The Relative Sensitivity Of Developmental And Immune Parameters. SOT, 49th Annual Meeting, Salt Lake City, 7-11 March 2010.

  37. Piersma A.H.. Lifestyle, occupation and pregnancy. Invited lecture, De Snoo van ’t Hoogerhuijs Foundation Annual Conference, Utrecht, 19 March 2010.

  38. Piersma A.H.. Proefdieronderzoek versus in vitro methoden in een brede context. Invited lecture. Symposium “Alternatieven voor dierproeven”, Aesclepios, Leiden, March 22, 2010.

  39. A.H.Piersma. Reproductive Toxicology Session Introduction. Netherlands Society of Toxicology Annual Meeting, 2 June 2010, Woudschoten, Woudenberg, The Netherlands.

  40. A.H.Piersma. The Embryonic Stem Cell Test, History and Progress. ICH Workshop on the Implementation of in vitro approaches in Developmental and Reproductive Toxicity Testing Strategies for Human Pharmaceuticals. 6 June 2010, Tallinn, Estonia.

  41. A.H.Piersma. Reproductive Toxicology and the GHSGlobally Harmonised System for Classification & Labelling in Europe. ContactGroep Chemie Invited Lecture, 10 June 2010, Den Bosch, The Netherlands

  42. Peter T Theunissen1,2, Esther de Jong1,3, Frederik J van Schooten2, Aldert H Piersma. Effects of triazole antifungal compounds on neural differentiation of murine embryonic stem cells as determined by flow cytometry. Teratology Society 50th Annual Conference, Louisville KY, USA, 26-30 June 2010.

  43. Ahmed M. Osman, Dorien A.M. van Dartel, Edwin Zwart, Marco Blokland, Jeroen L. A. Pennings, Aldert H. Piersma. Proteome profiling of mouse embryonic stem cells to define markers for cell differentiation and embryotoxicity. Free communication lecture, Teratology Society 50th Annual Conference, Louisville KY, USA, 26-30 June 2010;

  44. Piersma AH. The extended one-generation reproduction toxicity study: Improving hazard assessment and saving animals? Invited Lecture, Teratology Society 50th Annual Conference, Louisville KY, USA, 26-30 June 2010; ETS-TS Debate, European Teratology Society 38th Annual Conference, Barcelona, Spain, 5-8 September 2010.

  45. Esther de Jong, Marta Barenys, Aart Verhoef, Aldert H. Piersma. Relative Developmental Toxicity Of Triazoles In The Embryonic Stem Cell Test And The Whole Embryo Culture. Teratology Society 50th Annual Conference, Louisville KY, USA, 26-30 June 2010; European Teratology Society 38th Annual Conference, Barcelona, Spain, 5-8 September 2010.

  46. Mats F. Nilsson, Birger Scholz, Khalid M. Khan, Aldert H. Piersma and Lennart Dencker. Rodent Organogenesis Toxicology Ontology (ROTO): Linking Morphology to Embryotoxicity. Teratology Society 50th Annual Conference, Louisville KY, USA, 26-30 June 2010.

  47. Joshua F. Robinson, Aart Verhoef, Vincent van Beelen, Jeroen Pennings, Elaine M. Faustman, Aldert H. Piersma. Investigating toxicogenomic parallels between in vivo- and in vitro-based models used for developmental toxicology. European Teratology Society 38th Annual Conference, Barcelona, Spain, 5-8 September 2010.

  48. Sanne A.B. Hermsen, Evert-Jan van den Brandhof, Aldert H. Piersma. Relative embryotoxicity of 1,2,4-triazoles in the zebrafish embryotoxicity test and comparison with their in vivo potencies. European Teratology Society 38th Annual Conference, Barcelona, Spain, 5-8 September 2010.

  49. Ahmed M. Osman, Sjors H.W. Schulpen, Dorien A.M. van Dartel, Edwin Zwart, Marco Blokland, Jeroen L. A. Pennings, Aldert H. Piersma. Proteome profiling of mouse embryonic stem cells to define markers for cell differentiation and chemical-mediated embryotoxicity. Teratology Society 50th Annual Conference, Louisville KY, USA, 26-30 June 2010 and European Teratology Society 38th Annual Conference, Barcelona, Spain, 5-8 September 2010.

  50. A.H.Piersma. Innovations in regulatory reproductive toxicology. Conference lecture, AgChem Forum, Lyon, France, 8-9 September 2010.

  51. A.H.Piersma. The extended one generation reproductive toxicity study: principles, practice and politics. Najaarsvergadering Ned.Ver.Toxicologie, secties risk assessment & reproductive toxicology, TNO, Zeist, 23 november 2010.

  52. A.H.Piersma. IPAM TOX21 Conference lecture, Milan, Italy, 26 November 2010.

  53. Joshua F. Robinson, Peter Theunissen, Dorien van Dartel, Jeroen Pennings, Elaine M. Faustman, Aldert H. Piersma. Examination of MeHg-induced toxicogenomic response across in vivo and in vitro models used for developmental toxicology. SOT 50th Annual Meeting, Washington DC USA, 6-10 March 2011. Abstract. Prize winning presentation

  54. Esther de Jong, Marta Barenys, Sanne Hermsen, Aart Verhoef, Aldert Piersma. Comparison of the Embryonic Stem cell Test, the Whole Embryo Culture and the Zebrafish Embryo Test as alternative methods for developmental toxicity testing of triazoles. SOT 50th Annual Meeting, Washington DC USA, 6-10 March 2011. Abstract.

  1. Peter T Theunissen, Jeroen Pennings, Jos CS Kleinjans, Joshua F Robinson, Aldert H Piersma Methyl mercury induced perturbation of neural differentiation of murine embryonic stem cells over time described by transcriptomics. SOT 50th Annual Meeting, Washington DC USA, 6-10 March 2011. Abstract.

  2. Jochem Louisse, Esther de Jong, Johannes J.M. van de Sandt, Bas J. Blaauboer, Ruud A. Woutersen, Aldert H. Piersma, Ivonne M.C.M. Rietjens, Miriam Verwei. The use of in vitro toxicity data and physiologically based kinetic modeling to predict dose-response curves for in vivo developmental toxicity of glycol ethers in rat and man. SOT 50th Annual Meeting, Washington DC USA, 6-10 March 2011. Abstract.

  3. Maarke J.E. Roelofs, Martin van den Berg, Aldert H. Piersma, Majorie B.M. van Duursen Porcine adrenal cortex microsomes as in vitro alternative assay for screening on sex steroidogenesis toxicity. SOT 50th Annual Meeting, Washington DC USA, 6-10 March 2011. Abstract.

  4. George P. Daston, Robert E Chapin, Anthony R. Scialli, Aldert H. Piersma, Edward W. Carney, John M. Rogers, Jan M. Friedman. Validation of Screening Assays for Developmental Toxicity: An Exposure-Based Approach. SOT 50th Annual Meeting, Washington DC USA, 6-10 March 2011. Abstract.

  5. Aldert H. Piersma. Embryonic stem cell approaches for the derivation of biomarkers of embryotoxicity. British Toxicology Society Joint Spring Meeting with the Dutch Society of Toxicology, Durham University, UK, 27-30 March 2011.

  6. Aldert Piersma. Chemische veiligheid en proefdieren: het kan beter met minder. Gastcolumn op www.kennislink.nl, 5 Mei 2011: http://www.kennislink.nl/publicaties/chemische-veiligheid-en-dierproeve….

  7. Aldert H. Piersma. ES differentiation & molecular endpoints. AXLR8-2 Workshop “Innovative Toxicity Testing Strategies”, Berlin DE, 23-25 May 2011.

  8. Aldert H.Piersma. Male fertility and the need for alternative methods. The European perspective. HESI-DART Worskshop on Testicular Toxicity, Washongton DC, USA, 20 may 2011.

  9. Sanne A.B. Hermsen, Tessa E. Pronk, Evert-J. van den Brandhof, Leo T.M. van der Ven, Aldert H. Piersma. Class-specific gene expression changes in the zebrafish embryo after exposure to three glycol ether alkoxy acids and two 1,2,4-triazole antifungals. Teratology Society 51st Annual Meeting, San Diego CA, 25-29 June 2011; 39th Annual Conference of the European Teratology Society, Gent, Belgium, 4-7 September 2011, Abstract. Prize winning presentation

  10. Elisa CM Tonk, Aart Verhoef, Liset JJ de la Fonteyne, Henk van Loveren Aldert H Piersma Developmental Immunotoxicity in Male Rats After Juvenile Exposure To Di-octyltin dichloride.Teratology Society 51st Annual Meeting, San Diego CA, 25-29 June 2011, Abstract.

  11. Elisa CM Tonk, Aart Verhoef, Eric R Gremmer, Henk van Loveren, Aldert H Piersma. Relative Sensitivity of Reproductive and Immune Parameters in Juvenile Versus Adult Rats after Exposure to Di-(2-ethylhexyl) Phthalate (DEHP) Teratology Society 51st Annual Meeting, San Diego CA, 25-29 June 2011, Abstract.

  12. Joshua F. Robinson, Aart Verhoef, Aldert H. Piersma. Comparison of Retinoic Acid Teratogenesis between In Vivo and In Vitro Models by transcriptomics. Teratology Society 51st Annual Meeting, San Diego CA, 25-29 June 2011, Abstract.

  13. A.H. Piersma, E. Rorije, M.E.W. Beekhuijzen, R. Cooper, D.J. Dix, B. Heinrich-Hirsch, M.T. Martin, E. Mendez, A. Muller, M. Paparella, D. Ramsingh, E. Reaves, P. Ridgway, E. Schenk, L. Stachiw, B. Ulbrich, B.C. Hakkert. Combined retrospective analysis of 498 rat multi-generation reproductive toxicity studies: On the impact of parameters related to F1 mating and F2 offspring. Teratology Society 51st Annual Meeting, San Diego CA, 25-29 June 2011, Abstract.

  1. Elisa C Tonk, Aart Verhoef, Liset J de la Fonteyne, Ine D Waalkens-Berendsen, André P Wolterbeek, Henk van Loveren, Aldert H Piersma. Di-n-octyltin dichloride (DOTC) induced developmental immunotoxicity in male rats after juvenile exposure. 39th Annual Conference of the European Teratology Society, Gent, Belgium, 4-7 September 2011, Abstract.

  2. Peter T. Theunissen, Pennings JLA, Robinson JF, Claessen SMH, Kleinjans JCS, Piersma AH. Methylmercury induced perturbation of murine embryonic stem cell neural differentiation over time described by transcriptomics. 39th Annual Conference of the European Teratology Society, Gent, Belgium, 4-7 September 2011, Abstract.

  3. Sjors H W Schulpen, Dorien van Dartel, Jeroen Pennings, Joshua Robinson, Aldert H Piersma. Dose response analysis of four monophthalates in the murine embryonic stem cell test assessed by cardiomyocyte differentiation and gene expression. . 39th Annual Conference of the European Teratology Society, Gent, Belgium, 4-7 September 2011, Abstract.

  4. Esther de Jong, Lianne van Beek Aldert H. Piersma. The Effect Of Five Teratogenic Compounds On The Differentiation Of Murine Embryonic Stem Cells Into Osteoblasts. 39th Annual Conference of the European Teratology Society, Gent, Belgium, 4-7 September 2011, Abstract.

  5. Joshua Robinson, Aart Verhoef, Jeroen Pennings, Aldert Piersma. A Comparison between In Vivo and Whole Embryo Culture using Transcriptomics: Developmental Changes, Model Differences and Teratogenic Response. 39th Annual Conference of the European Teratology Society, Gent, Belgium, 4-7 September 2011, Abstract. Prize winning presentation

  6. Aldert Piersma. Juvenile Toxicity Approaches for Chemicals. Impact on OECD Guidelines. 39th Annual Conference of the European Teratology Society, Gent, Belgium, 4-7 September 2011, Lecture.

  7. Virissa Lenters, Bo AG Jönsson, Cathrine Thomsen, Lars Rylander, Gunnar Toft, Jens Peter Bonde, Aldert H Piersma, Roel Vermeulen, Dick Heederik. Interrelationships between blood serum levels of persistent organic pollutants and their determinants in inuit, polish and ukranian males (CLEAR project). 23d Conference of the International Society of Experimental Epidemiology, Barcelona, 13-16 September 2011, Abstract.

  8. Aldert H. Piersma. Rabbit versus Rat in Reproductive Toxicology Chemicals Legislation. DART Testing Strategies for Human Pharmaceuticals: Animal Models vs In-Vitro Approaches. DIA Pharma Event #11116, Leiden, the Netherlands, 10-11 October 2011, Lecture.

  9. Aldert H. Piersma. Implementation of new approaches in reproductive toxicity testing. CAAT-Johns Hopkins University, Baltimore MD, 28 October 2011, Seminar.

  10. Aldert H. Piersma. The need for alternative models for testicular toxicity testing. INVITROM Annual Meeting, 12 December 2011, Utrecht, the Netherlands, Invited Lecture.

  11. Aldert H. Piersma. The challenge of replacing animals in basic research. Minisymposium on human stem cell-derived cardiomyocytes for safety pharmacology: what does the future look like? UMCUniversitair Medisch Centrum Utrecht, 16 December 2011, Invited Lecture.

  12. Aldert H. Piersma. Test strategies and alternative approaches for developmental and reproductive toxicity from the European regulatory prespective. SOT 51st Annual Meeting, 11-15 March 2012, San Francisco CA USA, Invited Lecture.

  13. Aldert H. Piersma. Transcriptomics approaches towards biomarkers of embryotoxicity in the embryonic stem cell test. SOT 51st Annual Meeting, 11-15 March 2012, San Francisco CA USA, Invited Lecture.

  14. Sanne A.B. Hermsen, Tessa E. Pronk, Evert-Jan van den Brandhof, Leo T.M. van der Ven, Aldert H. Piersma. Concentration-response analysis of differential gene expression in the zebrafish embryotoxicity test following flusilazole exposure. SOT 51st Annual Meeting, 11-15 March 2012, San Francisco CA USA, abstract.

  15. Sjors H W Schulpen, Joshua Robinson, Jeroen Pennings, Aldert H Piersma. Potency ranking of monophthalates studied by transcriptomics in the mouse embryonic stem cell test. SOT 51st Annual Meeting, 11-15 March 2012, San Francisco CA USA, abstract.

  16. Joshua F. Robinson, Aart Verhoef, Jeroen Pennings, Aldert H. Piersma. A Transcriptomic Approach to Quantify Dose-Response Sensitivity to Phthalates in WEC. SOT 51st Annual Meeting, 11-15 March 2012, San Francisco CA USA, abstract.

  17. Joshua F. Robinson, Aart Verhoef, Jeroen Pennings, Aldert H. Piersma. A Comparison between In Vivo and Whole Embryo Culture using Transcriptomics: Developmental Changes, Model Differences and Teratogenic Response. SOT 51st Annual Meeting, 11-15 March 2012, San Francisco CA USA, abstract.

  18. Maarke J.E. Roelofs, Maria E. Flores Guillén, Martin van den Berg, Bas J. Blaauboer, Aldert H. Piersma, Majorie B.M. van Duursen. Endocrine disrupting effects of conazole fungicides on male sex steroidogenesis. SOT 51st Annual Meeting, 11-15 March 2012, San Francisco CA USA, Abstract.

  19. Aldert H. Piersma. Toxicogenomics in the Embryonic Stem Cell Test. XLR8 Berlin 11-13 June 2012. Invited Lecture.

  20. Aldert H. Piersma. Proteomic versus genomic markers for cell differentiation and embryotoxicity. Eurotox Stockholm, 17-20 June 2012. Invited Lecture.

  21. Aldert H. Piersma. Approaches for predicting developmental toxicity: an integrated testing strategy. Teratology Society 52d Annual Meeting, Baltimore MD, 23-27 June 2012, Invited Lecture.

  22. Joshua F. Robinson, Elisa C.M. Tonk, Aart Verhoef, Vincent A. van Beelen, Jeroen L.A. Pennings, Aldert H. Piersma. Dose-Response Analysis of Phthalate Effects on Gene Expression in Rat Whole Embryo Culture. 52nd Annual Meeting of the Teratology Society, Baltimore MD, 23-27 June 2012, Abstract.

  23. Joshua F. Robinson, Elisa C.M. Tonk, Aart Verhoef, Sanne A.B. Hermsen, Dorien A.M. van Dartel, Jeroen L.A. Pennings, Aldert H. Piersma. Triazole Induced Concentration-Related Gene Signatures in Rat Whole Embryo Culture. 52nd Annual Meeting of the Teratology Society, Baltimore MD, 23-27 June 2012, Abstract.

  24. Elisa CM Tonk, Aart Verhoef, André PM Wolterbeek, Ine DH Waalkens-Berendsen, Henk van Loveren, Aldert H Piersma. Developmental Immunotoxicity of Ethanol in EOGRTSExtended One Generation Reproductive Toxicity Study and Juvenile Exposure Designs. 52nd Annual Meeting of the Teratology Society, Baltimore MD, 23-27 June 2012, Abstract.

  25. Joshua F. Robinson, Elisa C.M. Tonk, Aart Verhoef, Sanne Hermsen, Dorien van Dartel, Jeroen L.A. Pennings, Aldert H. Piersma. Triazole Induced Concentration-Related Gene Signatures in Rat Whole Embryo Culture. 40th Annual Conference of the European Teratology Society, Linz, Austria, 2-5 September 2012, Abstract.

  26. Elisa C.M. Tonk, Joshua F. Robinson, Jeroen L. Pennings, Aldert H. Piersma. Transcriptomics applications in in vitro embryotoxicity assessment. 40th Annual Conference of the European Teratology Society, Linz, Austria, 2-5 September 2012, Abstract.

  27. Sanne A.B. Hermsen, Tessa E. Pronk, Evert-Jan van den Brandhof , Leo T.M. van der Ven, Aldert H. Piersma. Zebrafish embryotoxicity assessed by morphology and transcriptomics. 40th Annual Conference of the European Teratology Society, Linz, Austria, 2-5 September 2012, Abstract.

  28. van Dartel DAM and Piersma AH. The embryonic stem cell test combined with toxicogenomics as an alternative testing model for the assessment of developmental toxicity. Elsevier best RTX 2011 manuscript Award Lecture. 40th Annual Conference of the European Teratology Society, Linz, Austria, 2-5 September 2012, Abstract.

  29. Piersma A.H. Embryonic Stem Cell approaches towards biomarkers of embryotoxicity. EUSAAT, Linz, Austria, 5-8 September 2012, Invited Lecture.

  30. Theunissen P.T., Piersma A.H. Neural embryonic stem cell test. EUSAAT, Linz, Austria, 5-8 September 2012, Invited Lecture.

  31. Jeroen L.A. Pennings, Dorien A.M. van Dartel, Joshua F. Robinson, Tessa E. Pronk, Aldert H. Piersma An optimized biomarker set for developmental toxicity prediction using embryonic stem cell test transcriptomics. European Conference on Computational Biology,
    9-12 September 2012, Basel, Switzerland.

  32. Aldert H. Piersma. Developmental toxicology alternative assays. HESI-DART Symposium, Paris, France, 11-12 October 2012, Invited Lecture.

  33. Aldert H. Piersma. Toxicogenomic approaches in developmental toxicology. Toxicology Forum, Brussels, 23-25 October 2012, Invited Lecture.

  34. Aldert H. Piersma. IVTS London, UK, 20-21 November 2012, Invited Lecture.

  35. Aldert H. Piersma. Overview of Reproductive Toxicology. Beltox Edegem, Belgium, 3 December 2012, Invited lecture.